LOCUS       sequence-220943-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 2micron as a Type 7 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK082
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..138
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
       61 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      121 ccgccgggcg ttttttattg gtctggtctc atcgggaagt tcctattctc tagctagaaa
      181 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgatgacgc actttcaaaa
      241 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa
      301 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac
      361 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt
      421 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc
      481 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga
      541 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa
      601 agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat
      661 gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg
      721 gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa
      781 agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct
      841 ttgtta
//
