LOCUS       sequence-220962-        4292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL003473 MIP R
FEATURES             Location/Qualifiers
     source          1..4292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2180..2314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2217..2236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2271..2290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2353..2370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2604..2623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3283..4074,4075..4143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3906..3925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4144..4248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4288..4292,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgttggaccc agggggtaaa agagagtcaa ttttcggttc
      841 ggactttttg ccaccggacg atgtcattaa cggaagcatc ccggaccggg tgtacggcag
      901 tgtcatcttt caggaatctg ctggcagtgc acgagactac ggtggcgcag aagagttttc
      961 cctgctggag accctgttca acaactacac cgccgaagtt gccaacgtgt caacgacgga
     1021 agttccctac acaccgtacg tgcggagacc ggaaacctac atcgtgccga ttctgttcgc
     1081 tgctatattt atcgttggtg tgctgggcaa cggaacgctc atcatcgtgt tcctgcgaca
     1141 tcgagccatg cgaaatgtac ccaacacata tatattatcc ttagcgctgg cagacttgct
     1201 gctcatagtg acaactgttc catttacgtc aattctctac acggtggact catggccctg
     1261 gggtagctta ctctgcactt cgtccgagtt catcaaggac gtctcaatcg gggtgtcagt
     1321 attcacgctg accgctcttt ccggcgatcg gttcttcgcc atagttgatc cattgcgaaa
     1381 gttccacgct catggtggcg gacggaaagc aacgcggatg accatcacca ttgccgtcct
     1441 gatatggata ctggcgatta ttttcgccat tcctgccatc ctggggtcgc atatcaggac
     1501 ggaaaaagtg aacaaggacg ttacgattga cttttgctac ccatttccgg gcaagtgggg
     1561 cccggaatat gcccgcggtg tcgtactcgg aaagtttctg gcctactacg cggtgccact
     1621 gtgcataata gcaattttct acgcgctgat agcccgccat ctgatacaca gtgccaaaca
     1681 tgtgcctggg gaaacgcaag gaacggtgcg gcaggtcaaa gcgaggcgaa aagttgccgt
     1741 cactgtgcta gcgtttgtcg ttatatttgg gatctgcttc ctgccctcac atctcttcat
     1801 gctgtggttt tactacaacc caaattccca ggaagactat aatcactttt ggcacgtatt
     1861 gcggatagtg ggcttttgtt tgtcatttgc caacagttgt gccaatccgg ttgcgttgta
     1921 ttgcgtgagt ggagcgttcc gcaagcattt caataggtat ttgctgtgtg agggatcttc
     1981 cagtgctcga cggcgccgtg gggataacct gggccaccgg gacacatcaa tgacgagcac
     2041 catgtccagg cggtatccta gcaaacggat tggaacgcaa tcagtcagag tgtaagcggc
     2101 cgctagacta gtctagagaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2161 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2281 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattaattc
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4261 aaaagtgcca cctgcaagct tggctgtgga at
//
