LOCUS       sequence-221137-        8414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chemical induction of MAD1 localisation to a specific site in the 
            cell (by rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Kuijt TE, Omerzu M, Saurin AT, Kops GJ
  TITLE     Conditional targeting of MAD1 to kinetochores is sufficient to 
            reactivate the spindle assembly checkpoint in metaphase.
  JOURNAL   Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. 
            Epub 2014 Apr 4.
  PUBMED    24695965
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00412-014-0458-9"; journalName: "Chromosoma"; date: 
            "2014-10"; volume: "123"; issue: "5"; pages: "471-80"
COMMENT     Sequence Label: pcDNA5-eYFP-FKBP-MAD1-WT
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        482..685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     636..656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    687..705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    708..726
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     736..760
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      885..894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(891..893,894..896,897..1607)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(936..957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1197..1216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1544..1565
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1653..1673
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1677..1997
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(4233..4250)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4239..4463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4596..4615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4747..4794
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4802..5824
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4802..5824
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5954..6075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5991..6010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6045..6064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6124..6140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6124..6140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6137..6159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6148..6164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6172..6178,6179..6196,6197..6202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6217..6238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6355..6372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6526..7114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6606..6625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7285..8076,8077..8145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7908..7927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8146..8250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8325..8344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
       61 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      361 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      421 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      481 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      601 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      661 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga
      721 tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      781 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
      841 acttaagctt ggtaccgagc tcggatccgc tagcgctacc ggtcgccacc atggtgagca
      901 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      961 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1021 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1081 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1141 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1201 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1261 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1321 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1381 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1441 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1501 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1561 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggaggggatc
     1621 ggtggagctc caccggtggc ggccgctcta gagagaatct ttattttcag ggcgccggag
     1681 tgcaggtgga gactatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct
     1741 gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc tcccgggaca
     1801 gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag
     1861 gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct
     1921 atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg
     1981 agcttctaaa actggaaagc ttgggaggcg gtagtatgga agacctgggg gaaaacacca
     2041 tggttttatc caccctgaga tctttgaaca acttcatctc tcagcgtgtg gagggaggct
     2101 ctggactgga tatttctacc tcggccccag gttctctgca gatgcagtac cagcagagca
     2161 tgcagctgga ggaaagagca gagcagatcc gttcgaagtc ccacctcatc caggtggagc
     2221 gggagaaaat gcagatggag ctgagtcaca agagggctcg agtggagctg gagagagcag
     2281 ccagcaccag tgccaggaac tacgagcgtg aggtcgaccg caaccaggag ctcctgacgc
     2341 gcatccggca gcttcaggag cgggaggccg gggcggagga gaagatgcag gagcagctgg
     2401 agcgcaacag gcagtgtcag cagaacttgg atgctgccag caagaggctg cgtgagaaag
     2461 aggacagtct ggcccaggct ggcgagacca tcaacgcact gaaggggagg atctcggaac
     2521 tgcagtggag cgtgatggac caggagatgc gggtgaagcg cctggagtcg gagaagcagg
     2581 agctgcagga gcagctggac ctgcaacaca aaaaatgcca ggaagccaat cagaaaatcc
     2641 aggaactcca ggccagccaa gaagcaagag cagaccacga gcagcagatt aaggatctgg
     2701 agcagaagct gtccctgcaa gagcaggatg cagcgattgt gaagaacatg aagtctgagc
     2761 tggtacggct ccctaggctg gaacgggagc tgaagcagct gcgggaggag agcgcgcacc
     2821 tgcgggagat gagagagacc aacgggctgc tccaggaaga gctggaaggg ctgcagagga
     2881 agctggggcg ccaggagaag atgcaggaga cgctggttgg cttggagctg gagaacgaga
     2941 ggctgctggc caagctgcaa agctgggaga gactggacca gaccatgggc ctgagcatca
     3001 ggactccaga agacctttcc agattcgtgg ttgagctgca gcagagggag cttgccttga
     3061 aggacaagaa cagcgccgtc accagcagcg cccgggggct ggagaaggcc aggcagcagc
     3121 tgcaggagga gctccggcag gtcagcggcc agctgttgga ggagaggaag aagcgcgaga
     3181 cccacgaggc gctggcccgg aggctccaga aacgggtcct gctgctcacc aaggagcggg
     3241 acggtatgcg ggccatcctg gggtcctacg acagcgagct gaccccggcc gagtactcac
     3301 cccagctgac gcggcgcatg cgggaggctg aggatatggt gcagaaggtg cacagccaca
     3361 gcgccgagat ggaggctcag ctgtcgcagg ccctggagga gctgggaggc cagaaacaaa
     3421 gagcagacat gctggagatg gagctgaaga tgctgaagtc tcagtccagc tctgccgaac
     3481 agagcttcct gttctccagg gaggaggcgg acacgctcag gttgaaggtc gaggagctgg
     3541 aaggcgagcg gagtcggctg gaggaggaaa agaggatgct ggaggcacag ctggagcggc
     3601 gagctctgca gggtgactat gaccagagca ggaccaaagt gctgcacatg agcctgaacc
     3661 ccaccagtgt ggccaggcag cgcctgcgcg aggaccacag ccagctgcag gcggagtgcg
     3721 agcgactgcg cgggctcctg cgcgccatgg agagaggagg caccgtccca gccgaccttg
     3781 aggctgccgc cgcgagtctg ccatcgtcca aggaggtggc agagctgaag aagcaggtgg
     3841 agagtgccga gctgaagaac cagcggctca aggaggtttt ccagaccaag atccaggagt
     3901 tccgcaaggc ctgctacacg ctcaccggct accagatcga catcaccacg gagaaccagt
     3961 accggctgac ctcgctgtac gccgagcacc caggcgactg cctcatcttc aaggccacca
     4021 gcccctcggg ttccaagatg cagctactgg agacagagtt ctcacacacc gtgggcgagc
     4081 tcatcgaggt gcacctgcgg cgccaggaca gcatccctgc cttcctcagc tcgctcaccc
     4141 tcgagctctt cagccgccag accgtggcat aagatatcca gcacagtggc ggccgctcga
     4201 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4261 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4321 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4381 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4441 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4501 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4741 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     4801 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     4861 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     4921 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     4981 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5041 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5101 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5161 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5221 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5281 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5341 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5401 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5461 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     5521 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     5581 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5641 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5701 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     5761 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     5821 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5881 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5941 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6061 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6121 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6301 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8281 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8341 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8401 gcgagcaaaa ttta
//
