LOCUS       sequence-221184-       16525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette 
            to the mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 16525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector
FEATURES             Location/Qualifiers
     source          1..16525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2959..3040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2984..3003
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    join(3054..3072,3073..3095,3096..3114,3115..3137,3138..3156,
                     3157..3179,3180..3198,3199..3221,3222..3240,3241..3263,
                     3264..3282,3283..3305,3306..3324)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3357..3395
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3392..3416
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3529..3562)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3640..3688
                     /note="synthetic polyadenylation signal"
     polyA_signal    3697..4173
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    4185..4646
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4673..4706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4720..4729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4726..4728,4729..4731,4732..5445)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4726..4728,4729..4731,4732..5442)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4771..4792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5032..5051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5379..5400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5470..6058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5941..5952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6089..6296
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8826..9129
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9131..9408
                     /label=chicken beta-actin promoter
     intron          9410..10427
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10351..10373
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10435..10454
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    10515..10546
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    10592..10640
                     /note="synthetic polyadenylation signal"
     polyA_signal    10649..11125
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11160..11179)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11204..11252
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    11412..11443
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             join(11468..12085,12086..12091,12092..12127,12128..12130,
                     12131..12166,12167..12169,12170..12205,12206..12214)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    12257..12845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12728..12739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12876..13083
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    13097..13130
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        13157..13656
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13192..13215)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     13589..13608
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(14304..14320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14304..14320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14317..14339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14328..14344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14352..14358,14359..14376,14377..14382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14397..14418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14535..14552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14706..15294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14786..14805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15465..16256,16257..16325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16088..16107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16326..16430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16498..16516
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttaat
      481 tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg agagataatg
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     1681 ttgggggggg gctgtcccga gctcggtacc gccccatcct cactgactcc gtcctggagt
     1741 tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga tttagcaaga
     1801 tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg tgggggggtc
     1861 accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca tggccagtgg
     1921 gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc agccttcatg
     1981 atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc cagtcctact
     2041 gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat ccagacacca
     2101 tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt gtgctcctct
     2161 ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc cttaaggctg
     2221 ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat gtcccacgtc
     2281 catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca ctgaattggc
     2341 aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat aggacaaggg
     2401 ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg cagtttttcc
     2461 cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg ccccatccct
     2521 gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac
     2581 cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg
     2641 ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc
     2701 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     2761 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     2821 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     2881 tacatccctg ggggctttgg gggggggctg tcccggtacc cggggatcca ccggtatcga
     2941 ttcggccggc cgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3001 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgagtttac cactccctat
     3061 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3181 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3241 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3301 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta
     3361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3421 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     3481 gcggccccga attcgagctc ggtacccggg gatcaaattc aattaaatat aacttcgtat
     3541 agcatacatt atacgaagtt attcgcgagt gaataaatga aagcttgcag atcccctcag
     3601 caccatggct agcggcagcc tcggagtttg aatagataga ataaaatatc tttattttca
     3661 ttccatctgt gtgttggttt tttgtgtgag atctacgggt ggcatccctg tgacccctcc
     3721 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3781 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3841 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3901 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3961 gttcaagcga ttctcctgcc tcaggctccc gagttgttgg gattccaggc atgcatgacc
     4021 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4081 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4141 caggcgtgaa ccactgctcc cttccctgtc cttctgcctc agcgctgtaa gtctgcagaa
     4201 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact
     4261 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg
     4321 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg
     4381 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg
     4441 gccagctcat tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt
     4501 ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc
     4561 atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt
     4621 tttgccaagt tctaattcca tcagaaagct tttaattaac tgaatagcta acataacttc
     4681 gtatagcata cattatacga agttatcacg cgtgctagcg ccaccatggt gagcaagggc
     4741 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4801 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4861 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4921 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4981 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5041 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5101 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5161 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5221 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5281 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5341 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5401 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaacgcg ttacgtaaag
     5461 cttgtcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5521 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5581 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5641 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5701 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5761 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5821 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5881 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5941 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6001 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6061 cgacaccgag cgctgctcga ggcctcgact gtgccttcta gttgccagcc atctgttgtt
     6121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggacacg
     6301 tgggatcctc tagtctagag ccccatcctc actgactccg tcctggagtt ggatgagaga
     6361 taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat ttaccttctc
     6421 caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca ccgtccctgg
     6481 aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg cacggtgggt
     6541 gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga tttgacgatt
     6601 gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg acccagctgt
     6661 atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat caaacatgca
     6721 ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc catgctgggt
     6781 tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt tctgaacgct
     6841 gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc atgctgtcct
     6901 gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca aacatgtgtc
     6961 accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg gaatggcttt
     7021 acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc ccagagggtg
     7081 gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg caggcattca
     7141 aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc ctgcacatag
     7201 cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt tctatgattc
     7261 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     7321 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     7381 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     7441 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     7501 gggctttggg ggggggctgt ccctctagag ccccatcctc actgactccg tcctggagtt
     7561 ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat
     7621 ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca
     7681 ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg
     7741 cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga
     7801 tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg
     7861 acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat
     7921 caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc
     7981 catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt
     8041 tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc
     8101 atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca
     8161 aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg
     8221 gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc
     8281 ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg
     8341 caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc
     8401 ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt
     8461 tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     8521 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     8581 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     8641 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     8701 acatccctgg gggctttggg ggggggctgt ccctctagaa gtcgacggcg cgccctgcag
     8761 gaatctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8821 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8881 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8941 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9001 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9061 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     9121 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     9181 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     9241 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     9301 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     9361 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg gagtcgctgc
     9421 gacgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     9481 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     9541 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     9601 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     9661 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     9721 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     9781 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     9841 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     9901 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     9961 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
    10021 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
    10081 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
    10141 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
    10201 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
    10261 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
    10321 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
    10381 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
    10441 tgctggttat tgtgctgtct catcattttg gcaaagaatt gatttgatac cgcgggcccc
    10501 gcgtggacgg tacctaactt taaataatgc caattattta aagttacctc agcaccatgg
    10561 ctagcggcag cctcggagtt tgaatagata gaataaaata tctttatttt cattccatct
    10621 gtgtgttggt tttttgtgtg agatctacgg gtggcatccc tgtgacccct ccccagtgcc
    10681 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
    10741 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
    10801 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
    10861 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
    10921 gattctcctg cctcaggctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
    10981 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
    11041 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
    11101 aaccactgct cccttccctg tccttctgcc tcagctaatt gagtaggggg gaggctaact
    11161 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    11221 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
    11281 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
    11341 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
    11401 ggcaggccgt gtaactttaa ataatgccaa ttatttaaag ttaacgcgcg cgtccattta
    11461 attcaccatg tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa
    11521 tgaagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
    11581 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tggcaatcga
    11641 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
    11701 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
    11761 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
    11821 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
    11881 ccactttaca ctgggctgcg tattggagga tcaggagcat caagtagcaa aagaggaaag
    11941 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
    12001 ccatcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
    12061 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
    12121 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
    12181 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcaaa tggcgcgtta
    12241 cgtaaagctt gtcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
    12301 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
    12361 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
    12421 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
    12481 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
    12541 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
    12601 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
    12661 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
    12721 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
    12781 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
    12841 cccgcatcga caccgagcgc tgctcgaggc ctcgactgtg ccttctagtt gccagccatc
    12901 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    12961 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    13021 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    13081 ggacacgtgg gatccggtgc cagggcgtgc ccttgggctc cccgggcgcg tttgggcggc
    13141 cgcatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
    13201 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    13261 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    13321 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    13381 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    13441 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    13501 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
    13561 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
    13621 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaatcgccg ctagccgcca
    13681 ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
    13741 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
    13801 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
    13861 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
    13921 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
    13981 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
    14041 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
    14101 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
    14161 atcactggca aactgtgatg gacgacaccg tcagtgtaag tgctagcccc taggcctgaa
    14221 tcgaagttcc tattcttcaa aaggtatagg aacttcgagc tcgtcgactg cagaggcctg
    14281 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    14341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    14401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    14461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    14521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    14581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    14641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    14701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    14761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    14821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    14881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    14941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    15001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    15061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    15121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    15181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    15241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    15301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    15361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    15421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    15481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    15541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    15601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    15661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    15721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    15781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    15841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    15901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    15961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    16021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    16081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    16141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    16201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    16261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    16321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    16381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    16441 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    16501 aggcgtatca cgaggccctt tcgtc
//
