LOCUS       sequence-221199-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-X2 where the first consensus motif's lysine is mutated to 
            arginine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2R1
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(103..119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      122..369
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(138..156)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(391..409)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(430..446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(443..465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    454..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(478..484,485..502,503..508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    523..544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(661..678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaaggttc
       61 ttcaagaaga cctggatcat ctaagaggcc ttaaaagctt atcgataccg tcgacctcga
      121 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
      181 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
      241 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
      301 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
      361 ttaatttgcg gccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc
      421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      721 aaaggcggta atacggttat ccacagaat
//
