LOCUS       sequence-221204-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic acetylation substrate consisting of GFP fusion with 3 
            repeats of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X3
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..169
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             189..902
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(219..247)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     842..862
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
       61 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      121 accaagcata caatcaactc caagctggcc gctctagaac tagtggatcc cccgggctgc
      181 aggaattcat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      241 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      301 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      361 caacacttgt cactactttc acttatggtg ttcaatgctt ttcaagatac ccagatcata
      421 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      481 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      541 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      601 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      661 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      721 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      781 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
      841 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
      901 aaggttcttc aaaaaagacc tggatcatct aagaggcctg gctcatcaaa gagaccataa
      961 aagcttatcg ataccgtc
//
