LOCUS       sequence-221219-        5138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL017335 CAPA R
FEATURES             Location/Qualifiers
     source          1..5138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3026..3160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3063..3082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3117..3136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3199..3216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3370..3958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3450..3469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4129..4920,4921..4989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4752..4771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4990..5094)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(5134..5138,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgactttcca caacttcgac gacgttggca gtggcacgat
      841 agaatcgtac tggacaaacg ccacaaccac tgccaggacc acgaccaccc tcctcacagc
      901 acttctgacg acttccctcg ggacgtcatc gtcggaactt ggtgttgttc catcgaccgc
      961 cacatccacc ttgctggata ccggttggtg gactgactac ggaaatggca ccgcgctgga
     1021 gggatacgct ggcgttagtg cgaggtggcc attcaccggt gaaaactatt cgacccacgg
     1081 aaatcatgga ctcaccggag cggaggaaat cgtaccaccg tacgaccggt gtgatccaag
     1141 aaacgaaaac ttccagtgca ccgtgcagga gtttctcgag tacgcccggg gaccccagca
     1201 gatgccgctg tcgacagcgc tgttggtgac cattctcttc acggggatcc tcattaccgg
     1261 cgtcgtgggc aatttgattg tgtgtctggt aataattcga catcctcaga tgcacaccgc
     1321 caccaactac tatctgttca gtttggccgt atccgatttg atcctgctgc tgttgggtct
     1381 gccgtacgaa atcagcctct actggcacca gtacccgtac aacctggggt tggtgttctg
     1441 caaaatgcgc gctctcatgt cggaggcatc gacttacgtg tcggtgttga cgatagtggc
     1501 cttttcgatg gaacggtttc tggccatctg tcatccattg cacctgtaca ccatgtccgg
     1561 attgcagcgc ccggttcgca tcattgccgg cctctggatc gtcagtctct tcagcgcagt
     1621 gcctttcgcc gtgttcaccg atatcgatta cattctctac ccaccgaccc aagagaaaat
     1681 cgaggactcg gctttctgtg cgatgctgag caatccggaa ggaattcccc tgtgggagct
     1741 gtcgacatgc ctgtttttcg ccggcccgat ggtggtgatg attgttctct acggccggat
     1801 gggaatgcaa atccgctccc gaacgcaacg aaccgaggaa ctgggagtgc gaaatggttc
     1861 cattaacggt cctaaggtat ctcagtcgaa aaaggcgatt atccggatgc tagctgccgt
     1921 tgtgataacg ttctttgtgt gctgggcgcc gtttcacgcc cagaggttgc tctttctgta
     1981 cgcgcgggac tggcagcact tcaacacggt caatacgtgg ctgttttccg tcgcgggatg
     2041 gctgtactac gtttcgtgca ccgtcaatcc catcctgtac aacgtgatgt cccaccggta
     2101 tcgggtcgct ttccgggaga cactttgtgg ccggcggcga ggctttggca ccagctttgc
     2161 gcgggatcaa tcgagcttcc gcgaaacgac ggttgacgtg aacctgggct gcgagagctc
     2221 caagctgctc cgggctcgat cgatgatgca atctagcaaa cggtcccgct acaaaggcgc
     2281 tctccacacg tccaacagcg tgcgatacag tggcgaccat tacatccggc ggaactccct
     2341 gcagatgggc agccacattc cgggaagtcg cgcctcgctc tcaccaaaca tgccaagcga
     2401 cgtcgtggtc atgctggaaa acaagcttag cggtcgagca cgttgctaca ccacatcggc
     2461 ctcaacgctg acgacgtcca caacgacgac cacgatgacc accgccacca ccggggaaaa
     2521 catcctcaag gtacccctga taagcatcaa cggtggcacg ggctgtctcg tcaacaacaa
     2581 tgtaaatagc accaacaaca actccagcaa cgccaacaat aacgccatca ttcccaccga
     2641 caacagcaac aacatcaacc gtagcattag caaggaaaac aacctttcca acgcatccac
     2701 cccggttccg tcttcccggg cgtcgaccag cagcctgtca atcgaaatgg cactcgatac
     2761 cagcagcaaa caacctaacc ggaacgactt atccctgtct gaccgaacag acagcaacga
     2821 ttcccaccag caccatcctc aacagcatcc caatcctaat catcaattaa ttaacggcta
     2881 cggaagcgat aaaagcgcca gtcctgctgg aagtgctgtg atgcgtgaga catgtattta
     2941 agcggccgct agactagtct agagaaaaaa cctcccacac ctccccctga acctgaaaca
     3001 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3121 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccgggtacc gagctcgaat
     3181 taattcctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4141 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4201 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4261 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4321 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4381 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4441 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4501 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4561 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4621 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4681 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4741 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4801 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4861 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4921 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4981 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5041 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5101 tccccgaaaa gtgccacctg caagcttggc tgtggaat
//
