LOCUS       sequence-221242-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab3ab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab3ab
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1428..1448)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1456..1472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1488..1622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1512..1531)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1566..1585
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2061..2248,2249..2260)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2294..2310)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2294..2310)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2347..2367)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2347..2365)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2386..2402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2399..2421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2410..2426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2434..2440,2441..2458,2459..2464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2479..2500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2617..2634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2788..3376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2868..2887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3547..4338,4339..4407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4170..4189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4408..4512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4675..4696)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5045..5056,5057..5219)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5592..5608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5628..5648
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcttca gctacagaca accgttatgg
      781 acaaaaggag tcttctgacc agaactttga ttacatgttc aaaatcctaa tcatcgggaa
      841 tagcagcgtt gggaagacca gctttctgtt tcgctatgca gatgactcct tcacgccagc
      901 gttcgtaagc accgtcggca ttgacttcaa ggtgaagacc atctacagga acgacaagag
      961 gataaagctg caaatctggg acacagcggg gcaggagcgt tacagaacca tcaccaccgc
     1021 ttattaccgt ggagccatgg gattcatcct catgtacgac attaccaatg aggagtcttt
     1081 cgctgcagtg caggactggt ccacacagat taagacatac tcatgggata acgctcaggt
     1141 gcttctagta ggcaacaagt gtgacatgga ggatgagagg gtcgtggctt ctgaaagggg
     1201 cagacagctc tctgagcatc ttggttttga atatttcgaa gccagtgcca aggacaacat
     1261 taatgttaaa caaaccttcg agcggttggt ggacataatc tgcgagaaga tgtcagagag
     1321 cctggatgct gccgatcctg cagtgacggg agccaaacaa gggccgcagc tcacagagca
     1381 gcccgccgcc ccgcaccggg actgcgcctg ctgaaaatcc cacagctcaa ctttattata
     1441 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga
     1501 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1561 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1621 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     1681 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     1741 tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct
     1801 cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat
     1861 ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac
     1921 ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata
     1981 ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa
     2041 tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     2101 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     2161 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     2221 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     2281 agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     2341 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     2401 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2461 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2521 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2581 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2641 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2701 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3421 cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct tcacctagat
     3481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4501 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     4561 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4621 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4681 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4741 tatcagggcg atggcccacg ctggctaaga actcatcagc ctccccggtc catctaccca
     4801 cgtaccaatg caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt
     4861 tataggaatg gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg
     4921 ttggacccag gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata
     4981 tctgttcaga cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc
     5041 ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg
     5101 gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta
     5161 aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa
     5221 gaaaactaga gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac
     5281 aaggttttat cctgctttag aactgtttgt atttaattat caaactataa gacagacaat
     5341 ctaatgccag tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct
     5401 gattctcaaa attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag
     5461 tgctgaaaag cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga
     5521 ggctgcaaat agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat
     5581 gggccctcga gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag
     5641 aaaagttggc acgcgcagat tccgtgtggg cctggtaata tcttaccatt ttaagctatt
     5701 ttaagattag ggaaaaaaat taaataaaac tactttaaaa ttactttcct taaaaagcaa
     5761 catatttatt tagtttttgt ttaactggct caagattgta atattttact tttacatgtt
     5821 tttttaagta cctaaatgtg ttcaaaagta aagtatgtta ttgttttttt tttttaatgt
     5881 tagctaagct ttaaattgta atatattact tttaaaagtg actttttaaa aatgtaaata
     5941 aaaaagtaac tataaatgtg taatgcatgt tatttctgtg ggtttttaat tttcatctca
     6001 actgggctta caattttaac ataataaaaa tcaaatcaaa tcaaatcagg cccttatttg
     6061 tcacatacac tcttgtatac tgaaataaac taaatgttga gaaaaattta aatacaccaa
     6121 aataacgcat accttttttg taattaccta agtaaaacat ttagttcctt ttttaagtga
     6181 ctcaagattg taatctatta attgtaaaag ataaaactag tgatcaatct gacgtgcttc
     6241 tatgcagcct gtgagattaa ttgtgatgtt taattattaa acaatttaaa tggatcaaac
     6301 atttcacatg tgcttattgt gtccaacaaa accagcagaa gcacttaaaa atattgcatg
     6361 acaacatgag tttttaatgt aaattatata tacttcctaa acgaagttaa tcatatttct
     6421 aaaataataa tgtcattgaa atatttaaat aaatgataag ctttagttga ttcaacataa
     6481 aattgaaagc aataaggact tttttttttt acagcggtga agcaaaagca tgtaataatc
     6541 atataatata ataaataaat aaataaataa tggctcttgg tgcaaatatg aacactttaa
     6601 ataaatatag aataaatatg atcaataagt cataacgatg tgttttatgt tgattataat
     6661 ttaaacatgt ttctaacgca agttttaaaa actgttttaa agtattattt taagttataa
     6721 gatcaatttg actcaacatt ttgtagtgaa aatatgttgt gtaaaattaa tataatatac
     6781 actataaaaa tgcagggttc cacacaatac attaatgttg tcccaacaca aatcgattaa
     6841 gttaacaaat atatgtggat tgaacataaa aaattaagtt gtcccaatga aatctcaaga
     6901 attgtgttgt ttcagctcat tttaaataag tagttttaac aagtaaaaaa taacatattt
     6961 ttgagtgtgt atatatatat attacaaaat ataataatat aaaatgtaaa agatataaac
     7021 catgctacat atatttaaga gctattttgc acattacttt tttgcaagcc attttagtgt
     7081 tttttttaca aatatatatt agctaaatat acaatatctt aaatatacaa ttaatcagac
     7141 atgcaattaa tgcatttaac ctacataaaa atacatgatc aacatatgtt ttatgctgat
     7201 tacaactaaa tcaaacgtaa tgaaattaat ttataaaagc gatttaaata tgtcaatatt
     7261 ttaagttaaa atcacttgaa acctactata aatgaactta aattaaaaaa atctccttta
     7321 attccaatgc aacagtgttg ttttttcagc tgaggacccc cggtgcagat ggttggcccc
     7381 cggcccgcta ttctaaggaa gcctttctta attttagggc ggcgctgcat gcgctcagat
     7441 cagattcacg ggtggagagg gttattttct cttttttatt ttttcttgcc tgctcatatt
     7501 tgggcctgtg tgaaccccag gctccgcctc ggcccccagg agccgctctt atttaaaggg
     7561 gatcctcgcc ttcagtcttc gtcccagtgg aagcatcagt cttgtgataa caacttggtg
     7621 agtcctacag accagcgctc gactctaggc tttaaatgca ttgatttctc tggattagct
     7681 accgggagag ttttaacttt gcttattagt cttgtgctcg aactgctagg tttaaagagt
     7741 gttttctcta atgcatgcat gaagcgacag ctgaaaacca gaaatagagc gtttgtcctc
     7801 ctgttaaact ctagcctttc tttggcattt gtcagtccga gtcttcagct gccggcagga
     7861 gattgggttt cagtaacact tgctgagcac aaactacacg tgctttcttc agatttaatt
     7921 cattattaat cattctagat ttaaatgatg ttcttactca ttgctgatta ttttagcctt
     7981 gcggagcaga ttaaatgagc acgtgtttca ttaagattga tttaaatata attaatattt
     8041 caccactgta gttttaaaat tgccttctaa gtcatggttg atttttaata ggtatcggct
     8101 ctaattagat tactattttg gtttttaaat gttatttcta tttcatttgt aggctcgttt
     8161 ttaatgtgat tttattagat tctagtttta aaatcatctc ttagccttaa tgagcagact
     8221 gaatgaacat gtttcattaa gattaacttc attataatga attatttaat cattctagtg
     8281 ggttataaat cagctctatt taaattaatt gtcgtgttta tttctattta atttttgatt
     8341 attttttcag ggtcgttttt atgtgatttt ttcccgtttc taattttaaa ctaattgttt
     8401 gatttttata tgattcgact ctttttaaat gaattgtttt gcttttttta ttgagcatat
     8461 atatattata caatttcaat caacaattgt tgtgcttttt aaatgttgtt tctatttcat
     8521 ttctagggtc gattttattc gattctagtt ttaaaatcgt gatttttaac ttctaacctt
     8581 actgagcaga ctgaatgaac atgtttcatc aagattaact tcattatagt taattattga
     8641 atcgtgctag tggtttataa atcagctcta tttaaattaa tcatcatcat tatttctatt
     8701 taatttttat tatttcttta ggattatttt tatgtgattt ttttcacgtt ctagttttaa
     8761 actcaatgct cattactgct cacccttgct gagcagtctt tcgttaagat taaattaatt
     8821 aataaccatt ctagttttaa aacagtgtct taaggcatag ctgatatttt acaagtttac
     8881 tatttaatat atctcctttc tttttttagg gtcgatttca tctgatttga ttaattttta
     8941 ttatcttcac taagcttttt gttctaggtt ttattctcag tattattact gttatatttg
     9001 aactgaattt ctcctcatag tttattcatt ttatttattt attttttgtt tattcgctat
     9061 tttgtatttt agtttttaat tagaaatttt attagaatcg ttttgaacta gtttttatgc
     9121 actctaacat taatattaaa tcataactat tatttttatt ataatttatt tgtgtttatc
     9181 tttttgtgta gctttcatgt gtttttattt tagttttatt atgtttgcat tgaatttttt
     9241 ggactagttt atataacgat atacagaaaa gccatgacaa caaatgtttt tataatacct
     9301 attaagtata agctaatcag atttccacta ttctgttcaa agttaacatt attagccatt
     9361 ctgatggatc atttgacatt tgtcatatgt ctttatgaac actgctcttt cagatgccac
     9421 ttttaaagct gttttttaca gtaaatgaac gtttattatt atgacattat ttcctaaggc
     9481 ctctagagta cagcaggatg tggcgtgtgc attaccgtgt aattatgctg actgtaaatg
     9541 tgcatttata gactgtcagc atgtctgact gtgcgctgtt gtgattggtc ctgcacagac
     9601 caag
//
