LOCUS       sequence-221247-        4898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5570885 Cardioacceleratory peptide receptor
FEATURES             Location/Qualifiers
     source          1..4898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2786..2920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2823..2842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2877..2896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2959..2976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3130..3718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3210..3229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3889..4680,4681..4749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4512..4531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4750..4854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4894..4898,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgctgtcact cgttgtctgg gcttaccttc gccattacgc
      841 caccacacat ttgcttgagc aacattccag cctcaacgaa cctcagcaag aattcctcga
      901 taagctcggg acccacttgg acacccacca tcatgtcgct accgtgcaag gcaaccagag
      961 tcatcaatat tggcgtaatg ataccaccac ttctggctgc agagtgaatt tgacctcgtt
     1021 cttccgtcat atcccgctga gtgctcgacg aggtgtcaac ttgtggactt gggtggactt
     1081 gggcggctgg attggggtcg taaatcttaa tcactgctgt gccgccaacg agggtcctgc
     1141 ttctactatt atgagatttg gtgataacag cagcaatttg tcgtcgttgc taaccgaaca
     1201 gagtgctaac attgaagtga gtgccgcagg aagcacttcc ggtggtcagg tgtcgtcttt
     1261 ggtggatcac gtcgcctccg ccacgagtgc cacgagtgct agtgagacaa acattaacga
     1321 cagtgtggtg ggtgtagttg ggcgtccttc cggtgtcggt ggggcagagg atgcgtttag
     1381 tatgaacatc actccggcac tttctcaggt tttattagcg ccaacctcgg cgagcggttc
     1441 cggagccatc atatcggttg ctgctgccac ggtcaccaaa gtcggtcctg ccaatgacaa
     1501 cgtgacgagt gaggagataa attcatttta tttttatgag catatttttt tttctttcca
     1561 gactgaacaa tttgcagtat tatgggcact tttcaccgtg attgtgctgg gaaactcggc
     1621 cgtgctggtc acgctgctgc tgaacaagaa ccgcaagtcc cgaatgaact tcttcatcaa
     1681 acagttggcg atagcagatt tatgcgtggg tttgctcagc gtgctgacgg atattatttg
     1741 gcggatgacg gtatcctgga aggccggaaa cgtcgcttgc aaggcaatcc gcttcgccca
     1801 ggccagcgtc acctacgcat caacctatgt cctggtggct ctcagcatcg accggtacga
     1861 tgccatcacg catccgatga acttctccgg ctgttggaat cgcgcacgtc ggttggtagc
     1921 agcagcctgg agtttaagtg cacttttctc acttccaatt ttttatctct atcaggagaa
     1981 atcaatacag ggaaggaaac agtgctggat tgacctgggc gaggcttggc ggtggcaggt
     2041 ttacatgtgc tgggtggcca gttccctgtt tgtcctgcca gcactgatca tctcagcatg
     2101 ttatgcaatc atcgtcaaga caatttgggc caagggagca attatgggac caatcgatcg
     2161 aactcgaaat ggaatggcag atttagccag cagacgggcg agctccaggg gcatcattcc
     2221 aaaggcaaaa gtaaaaacag tcaagatgac tattgtgata gtgatagtat tcgtcctctg
     2281 ctggtcgccg tacatcgtgt ttgacctgtt gcaagtgttt ggccagattc cggacacgca
     2341 aaccaatatt gcgattgcaa cgtttatcca aagcctggcg ccgctcaact cggctgccaa
     2401 cccactcatc tactgtctct tctcgacgca agtgtgtcga atgataaaac gacttccccc
     2461 atttcggtgg ctgctgtctt cggaatggtg ctgtaaaaac ccggacggtt cctcgggccg
     2521 aggtccactt cgcaacggta tggtgctaaa cgggggccaa aggctgcaga acaacaacag
     2581 tagcgactcg atgcgaacgt tgaccacctc gctgacgatt tcacagcggt cctgcatcag
     2641 gccatcgcgg gtcgtcatcg tcgagcgacc gaaagttgcc ctcgccatgt ccgaagtttg
     2701 agcggccgct agactagtct agagaaaaaa cctcccacac ctccccctga acctgaaaca
     2761 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2881 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccgggtacc gagctcgaat
     2941 taattcctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4861 tccccgaaaa gtgccacctg caagcttggc tgtggaat
//
