LOCUS       sequence-221272-        3240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 backbone with E. coli CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Wright AV, Doudna JA
  TITLE     Protecting genome integrity during CRISPR immune adaptation.
  JOURNAL   Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 
            10.1038/nsmb.3289. Epub 2016 Sep 5.
  PUBMED    27595346
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3289";
            journalName: "Nat Struct Mol Biol"; date: "2016-10"; volume: "23"; 
            issue: "10"; pages: "876-883"
COMMENT     Sequence Label: pCRISPR
FEATURES             Location/Qualifiers
     source          1..3240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1421..2009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1501..1520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2180..2971,2972..3040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2803..2822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3041..3145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3213..3231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctaccttttt aatcaatgga
      421 ttaagtactc tttaacataa tggatgtgtt gtttgtgtga tactataaag ttggtagatt
      481 gtgactggct taaaaaatca ttaattaata ataggttatg tttagagtgt tccccgcgcc
      541 agcggggata aaccgagcac aaatatcatc gctcaaacca cttacgggtg ttccccgcgc
      601 cagcggggat aaaccgcctc gctgtaaatt ccaaaaacga tctctatagt gttccccgcg
      661 ccagcgggga taaaccgaca gtaccggagt ttgacggtgc caacggcggg tgttccccgc
      721 gccagcgggg ataaaccgac aatcagggaa cgattgttga cactgtaaaa gtgttccccg
      781 tgctagcggg gataaaccga cctgaacgaa acgcaaaata gtttctttat ggtgttcccc
      841 gcgccagcgg ggataaaccg tggctgtggc tttgtttttt gctctgatat atgtgttccc
      901 cgcgccagcg gggataaacc ggcgtccgtc aaagaggaag ccaaagccat gcagtgttcc
      961 ccgcgccagc ggggataaac cggcgaccac gcagagcggc gacaacctga cgttgtgttc
     1021 cccgcgccag cggggataaa ccgctatagc agcactccac cagctcgagc agcaggtgtt
     1081 ccccgcgcca gcggggataa accgcaaaaa aataatatcc ggcagtctgt acggtagtgt
     1141 tccccgcgcc agcggggata aaccgtcggt ttgcgcgtat tgttgttcgg ggtctatgtg
     1201 ttccccgcgc cagcggggat aaaccgtagg taaatcacag ctatttgata agggcgtgtg
     1261 tgttccccgc gccagcgggg ataaaccgga gcctgacgag actgctgagg ccgttctgtc
     1321 gagttccccg cgccagcggg gataaaccac catataacca catgtgagca aaaggccagc
     1381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3181 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
