LOCUS       sequence-221318-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-GFP
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(12..40)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     635..655
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
       61 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      121 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
      181 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
      241 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      301 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      361 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      421 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      481 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      541 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      601 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
      661 ctgggattac acatggcatg gatgaactat acaaataggg cgcgccactt ctaaataagc
      721 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
      781 caa
//
