LOCUS       sequence-221323-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein mutated from lysine to 
            arginine at site 166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-K166R-GFP
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..27
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11..27
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     85..101
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     85..101
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
       61 gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct gcagaatgag
      121 gctgaaacgg tttgaataat taggaaagta tgtttttaat aaagaaattc tatgttcagg
      181 aattttgtat atactttgta atgaatgaga acttagttgg cttcaaactt tttcgtttaa
      241 catgattatt tttcttgttc gactaagata ttcccacatg gacaagtgcc acagattaat
      301 atatgaatac aataaaataa ctagtgtaat ttgaaaataa aagttgatga gagggacagg
      361 gacttgagtc ctattcaaag tgaaatattt tagttatgag ttttctttcc aaactttccc
      421 aaatacgaaa atcaacgact gcatcaaaag cccaagtgca agatccatta cccaagaaga
      481 atgacgaaga gtattccttg ttacccaaaa attacataag agacgaagat cctgcagtaa
      541 aaagattgaa ggagctgagg cggcaggaac tgttaaagaa tggtgctttg gctaaaaaaa
      601 gtggtgtaaa acggaaacgt ggcacctcat ctggatctga gaaaaagaaa atagaaagga
      661 atgacgatga tgaaggtggc cttggaatta ggtttaagag gtctattgga gcaagtcatg
      721 cgccactcaa gccagttgta aggaagaaac ctgaacctat caaaaagatg tcatttgaag
      781 agctaatga
//
