LOCUS       sequence-22133-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GPD 25QDProGFP p416
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(205..221)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(205..221)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(227..490)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(310..333)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ccggtgacgc tcctcgccct tgctcaccag ggatcccccg ggctgcagtt gttgctgttg
       61 ctgctgttgt tgctgttgct gctgttgttg ctgttgctgc tgttgttgct gttgctgctg
      121 ttggaagctt ttgagggact cgaaggcctt catcagcttt tccagggtcg ccatggcggt
      181 cgacctcgag ggggggcccg gtacgatcca ctagttctag aatccgtcga aactaagttc
      241 tggtgtttta aaactaaaaa aaagactaac tataaaagta gaatttaaga agtttaagaa
      301 atagatttac agaattacaa tcaataccta ccgtctttat atacttatta gtcaagtagg
      361 ggaataattt cagggaactg gtttcaacct tttttttcag ctttttccaa atcagagaga
      421 gcagaaggta atagaaggtg taagaaaatg agatagatac atgcgtgggt caattgcctt
      481 gtgtcatcat ttactccagg caggttgcat cactccattg aggttgtgcc cgttttttgc
      541 ctgtttgtgc ccctgttctc tgtagttgcg ctaagagaat ggacctatga actganggtt
      601 ggtgaagaaa acaatatttt ggtgctggga ttcttttttt ttctggatgc cagcttaana
      661 agcgggctcc attatattta gtggatgcca ggaataaact gttcacccag acacctacga
      721 tgttatatat tctgtgtaac ccgcccccta ttttgggcat gtacgg
//
