LOCUS       sequence-22148-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG25QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..23
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(198..226)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actacaagga cgacgatgac aagctggcga ccctggaaaa gctgatgaag gccttcgagt
       61 ccctcaaaag cttccaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc
      121 aacagcaaca acagcagcaa cagcaacaac tgcagcccgg gggatccctg agtaaaggag
      181 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      241 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      301 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttga
      361 cttggggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
      421 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      481 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      541 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      601 atatttcaca caatgtatac atcactgcag acaaacaaaa gaatggaatc aaagctcatt
      661 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      721 atactccaat tgg
//
