LOCUS       sequence-22154-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: MET FLAG46QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(31..278)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     244..262
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(328..348)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggtcgacggt atcgataagc ttgatcggcc gcaaattaaa gccttcgagc gtcccaaaac
       61 cttctcaagc aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa
      121 aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat
      181 agggacctag acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg
      241 agggcgtgaa tgtaagcgtg acataactaa ttacatgact cgagctattt gtatagttca
      301 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc
      361 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
      421 aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt
      481 ccatcttcaa tgttgtgtct aattttgaaa tgagctttga ttccattctt ttgtttgtct
      541 gcagtgatgt atacattgtg tgaaatatag ttgtattcca atttgtgtcc aagaatgttt
      601 ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca
      661 aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc
      721 tgtacataac cttcgggcat ggcactcttg aaaaagtcat gccgtttcat atgatctggg
      781 tatcttgaaa agcattgaac accccaagtc aaagtagtga caagtgttgg ccatggaaca
      841 ggtagttttc cagtagtgc
//
