LOCUS       sequence-221604-        7953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are 
            deleted).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, 
            Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
  TITLE     A TPR domain-containing N-terminal module of MPS1 is required for 
            its kinetochore localization by Aurora B.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. 
            Epub 2013 Apr 8.
  PUBMED    23569217
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201210033"; journalName: "J Cell Biol"; date: 
            "2013-04-15- 15"; volume: "201"; issue: "2"; pages: "217-31"
COMMENT     Sequence Label: pCDNA5-LAP-MPS1-d200
FEATURES             Location/Qualifiers
     source          1..7953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        104..483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        484..687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     638..658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    689..707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    710..728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     738..762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      887..896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(893..895,896..898,899..1609)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(938..959)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1199..1218)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1546..1567
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1655..1675
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1688..1732
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(3774..3791)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3780..4004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4137..4156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4288..4335
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4343..5365
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4343..5365
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5495..5616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5532..5551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5586..5605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5665..5681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5665..5681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5678..5700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5689..5705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5713..5719,5720..5737,5738..5743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5758..5779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5896..5913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6067..6655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6147..6166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6826..7617,7618..7686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7449..7468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7687..7791)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7866..7885)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
       61 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      661 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
      721 gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      781 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
      841 aaacttaagc ttggtaccga gctcggatcc gctagcgcta ccggtcgcca ccatggtgag
      901 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      961 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1021 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1081 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1141 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1201 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1261 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1321 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1381 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1441 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1501 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1561 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggagggga
     1621 tcggtggagc tccaccggtg gcggccgctc tagagagaat ctttattttc agggcgccgc
     1681 caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcggaggtgg
     1741 aggtactagt ctcgagaaga atttatcagc atctacggta ttaactgccc aagaatcatt
     1801 ttccggttca cttgggcatt tacagaatag gaacaacagt tgtgattcca gaggacagac
     1861 tactaaagcc aggtttttat atggagagaa catgccacca caagatgcag aaataggtta
     1921 ccggaattca ttgagacaaa ctaacaaaac taaacagtca tgcccatttg gaagagtccc
     1981 agttaacctt ctaaatagcc cagattgtga tgtgaagacg gacgattcag ttgtaccttg
     2041 ttttatgaaa agacaaacct ctagatcaga atgccgagat ttggttgtgc ctggatctaa
     2101 accaagtgga aatgattcct gtgaattaag aaatttaaag tctgttcaaa atagtcattt
     2161 caaggaacct ctggtgtcag atgaaaagag ttctgaactt attattactg attcaataac
     2221 cctgaagaat aaaacggaat caagtcttct agctaaatta gaagaaacta aagagtatca
     2281 agaaccagag gttccagaga gtaaccagaa acagtggcaa tctaagagaa agtcagagtg
     2341 tattaaccag aatcctgctg catcttcaaa tcactggcag attccggagt tagcccgaaa
     2401 agttaataca gagcagaaac ataccacttt tgagcaacct gtcttttcag tttcaaaaca
     2461 gtcaccacca atatcaacat ctaaatggtt tgacccaaaa tctatttgta agacaccaag
     2521 cagcaatacc ttggatgatt acatgagctg ttttagaact ccagttgtaa agaatgactt
     2581 tccacctgct tgtcagttgt caacacctta tggccaacct gcctgtttcc agcagcaaca
     2641 gcatcaaata cttgccactc cacttcaaaa tttacaggtt ttagcatctt cttcagcaaa
     2701 tgaatgcatt tcggttaaag gaagaattta ttccatatta aagcagatag gaagtggagg
     2761 ttcaagcaag gtatttcagg tgttaaatga aaagaaacag atatatgcta taaaatatgt
     2821 gaacttagaa gaagcagata accaaactct tgatagttac cggaacgaaa tagcttattt
     2881 gaataaacta caacaacaca gtgataagat catccgactt tatgattatg aaatcacgga
     2941 ccagtacatc tacatggtaa tggagtgtgg aaatattgat cttaatagtt ggcttaaaaa
     3001 gaaaaaatcc attgatccat gggaacgcaa gagttactgg aaaaatatgt tagaggcagt
     3061 tcacacaatc catcaacatg gcattgttca cagtgatctt aaaccagcta actttctgat
     3121 agttgatgga atgctaaagc taattgattt tgggattgca aaccaaatgc aaccagatac
     3181 aacaagtgtt gttaaagatt ctcaggttgg cacagttaat tatatgccac cagaagcaat
     3241 caaagatatg tcttcctcca gagagaatgg gaaatctaag tcaaagataa gccccaaaag
     3301 tgatgtttgg tccttaggat gtattttgta ctatatgact tacgggaaaa caccatttca
     3361 gcagataatt aatcagattt ctaaattaca tgccataatt gatcctaatc atgaaattga
     3421 atttcccgat attccagaga aagatcttca agatgtgtta aagtgttgtt taaaaaggga
     3481 cccaaaacag aggatatcca ttcctgagct cctggctcat ccatatgttc aaattcaaac
     3541 tcatccagtt aaccaaatgg ccaagggaac cactgaagaa atgaaatatg ttctgggcca
     3601 acttgttggt ctgaattctc ctaactccat tttgaaagct gctaaaactt tatatgaaca
     3661 ctatagtggt ggtgaaagtc ataattcttc atcctccaag acttttgaaa aaaaaagggg
     3721 aaaaaaatga gtcgagcatg catctagagg gcccgtttaa acccgctgat cagcctcgac
     3781 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3841 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3901 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3961 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4021 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4081 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4141 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4201 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4261 tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga
     4321 aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     4381 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     4441 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     4501 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     4561 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     4621 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     4681 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     4741 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     4801 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     4861 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     4921 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     4981 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     5041 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     5101 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     5161 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     5221 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     5281 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     5341 cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt
     5401 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5461 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5581 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5641 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5701 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5761 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5821 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5881 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6481 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6601 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6661 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6721 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6781 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6841 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6901 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6961 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7021 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7081 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7141 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7201 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7261 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7321 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7381 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7441 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7501 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7561 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7621 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7681 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7741 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7801 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7861 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7921 ttggaggtcg ctgagtagtg cgcgagcaaa att
//
