LOCUS       sequence-221609-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This lentiviral plasmid expresses MCV tumor T antigens (truncated LT
            and small T) using the viral NCCR (non-coding control region). The 
            sequence was amplifeied from MCV.R17a and truncated LT=330 aa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  TITLE     Lentiviral expression plasmid for MCV T antigens via MCV promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.puro.MCV.ER.RAZ.1
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    493..726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             911..955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1222..1338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3261..3849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3314..3334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3732..3743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3863..4350
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3898..3921)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4283..4302
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4371..4970
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4371..4390)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4867..4887
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5108..5341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5413..5534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5450..5469)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5504..5523
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5574..5709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5729..5748)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5730..5748)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5758..5775)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5789)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5916..6371
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6003..6022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6213..6234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6397..6501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6502..6570,6571..7362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6720..6739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7533..8121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8022..8041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8275..8292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8409..8430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8445..8450,8451..8468,8469..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8488..8510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8507..8523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8507..8523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8542..8562
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8544..8562
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8590..8816
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8817..8997
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9044..9169
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      361 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      421 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      481 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      541 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      601 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
      661 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
      721 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
      781 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
      841 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
      901 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
      961 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1021 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1081 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1141 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1201 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     1261 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1321 tacaaaattc aaaattttat cgatcctgaa aaataaataa ggatacttac tcttttaatg
     1381 tcctcctccc tttgtaagag aaaaaaaagc ctccgggcct cccttgttga aaaaaagtta
     1441 agagtcttcc gtctccctcc caaacagaaa gaaaaaaagt tttgtttatc agtcaaactc
     1501 cgcctctcca ggaaatgagt caatgccaga aaccctgcag caataaaagt tcaatcatgt
     1561 aaccacaact tggctgccta ggtgactttt ttttttcaag ttggcagagg cttggggctc
     1621 ctagcctccg aggcctctgg aaaaaaagag agaggcctct gaggcttaag aggcttaatt
     1681 agcaaaaaag gcagtatcta agggcagatc ccaagggcgg gaaactgcag tataaaaacc
     1741 actccttagt gaggtagctc atttgctcct ctgctctttc tgcaaactcc ttctgcatat
     1801 agacaagatg gatttagtcc taaataggaa agaaagagag gctctctgca agcttttaga
     1861 gattgctcct aattgttatg gcaacatccc tctgatgaaa gctgctttca aaagaagctg
     1921 cttaaagcat caccctgata aagggggaaa tcctgttata atgatggaat tgaacaccct
     1981 ttggagcaaa ttccagcaaa atatccacaa gctcagaagt gacttctcta tgtttgatga
     2041 ggtcagtaca aaatttcctt gggaagaata tggaacttta aaggattata tgcaaagtgg
     2101 atataatgct agattttgca gaggtcctgg gtgcatgctt aagcaactta gagattctaa
     2161 gtgcgcttgt attagctgta agttgtctcg ccagcattgt agtctaaaaa ctttaaagca
     2221 aaaaaactgt ctgacgtggg gagagtgttt ttgctatcag tgctttattc tttggtttgg
     2281 atttcctcct acttgggaaa gttttgactg gtggcaaaaa actttagaag aaactgacta
     2341 ctgcttactg catctgcacc ttttctagac tcctacttcc ttcctctgta agtattagat
     2401 atggaaaagt ctataaggca aaatatcaaa gaaaggttat ttatgacaga ttttctgtac
     2461 tttcccatct aggttgacga ggcccctata tatgggacca ctaaattcaa agaatggtgg
     2521 agatcaggag gattcagctt cgggaaggca tacgaatatg ggcccaatcc acacgggacc
     2581 aactcaagat ccagaaagcc ttcctccaat gcatccaggg gagcccccag tggaagctca
     2641 ccaccccaca gccagagctc ttcctctggg tatgggtcct tctcagcgtc ccaggcttca
     2701 gactcccagt ccagaggacc cgatatacct cccgaacacc atgaggaacc cacctcatcc
     2761 tctggatcca gtagcagaga ggagaccacc aattcaggaa gagaatccag cacacccaat
     2821 ggaaccagtg tacctagaaa ttcttccaga acggatggca cctgggagga tctcttctgc
     2881 gatgaatcac tttcctcccc tgagcctccc tcgtcctctg aggagcctga ggagcccccc
     2941 tcctcaagaa gctcgccccg gcagcccccg tcttcctctg ccgaggaggc ctcgtcatct
     3001 cagtttacag atgaggaata cagatcctcc tccttcacca ccccgaagac ccctcctcca
     3061 ttctcaagaa agcgaaaatt tggggggtcc cgaagctctg caagctctgc tagttcagca
     3121 agttttacaa gcactccacc aaagccaaaa aagaacagag aaactcctgt tcctactgat
     3181 tttcctattg atctttctga ttatcttagc catgctgtat atagtaatta ggatatccag
     3241 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     3301 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3361 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3421 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3481 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3541 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3601 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     3661 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3721 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3781 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3841 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     3901 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     3961 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4021 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4081 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4141 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     4201 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg ggcgggcgcc
     4261 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     4321 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     4381 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4441 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4501 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4561 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4621 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4681 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4741 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4801 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4861 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4921 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     4981 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
     5041 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5101 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     5161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5341 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     5401 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5521 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     5581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5641 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5701 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5761 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5821 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5881 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     5941 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6001 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6061 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6121 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6181 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6241 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6301 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6361 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6721 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7021 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7441 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7501 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7561 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7621 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7681 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7741 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7801 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7861 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7921 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7981 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8041 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8101 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8161 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8221 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8281 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8341 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8521 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     8581 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     8641 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     8701 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     8761 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     8821 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8881 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8941 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     9001 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9061 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     9121 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtc
//
