LOCUS       sequence-22200-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Popov N, Wanzel M, Madiredjo M, Zhang D, Beijersbergen R, Bernards 
            R, Moll R, Elledge SJ, Eilers M
  TITLE     The ubiquitin-specific protease USP28 is required for MYC stability.
  JOURNAL   Nat Cell Biol. 2007 Jul . 9(7):765-74.
  PUBMED    17558397
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Jul . 9(7):765-74."
COMMENT     Sequence Label: pRetrosuper USP28 shRNA-1
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..103)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(88..104)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(122..538)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(138..154)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(170..192)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctgtatgag acacagatcc ccggagtgag attgaacaag attcaagaga tcttgttcaa
       61 tctcactcct ttttggaaaa gcttatcgat accgtcgacc tcgagagatc gatctaattc
      121 cggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
      181 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
      241 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
      301 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
      361 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
      421 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
      481 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct
      541 aattttcaga caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc
      601 gctgcagcag acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacggag
      661 gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatcctgac ggggtcggat
      721 tccacatcga ctcccttcct caggtcgggc cacaaaaacg gcccccaaag tccctgggac
      781 gtctc
//
