LOCUS       sequence-222028-       11228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites
            ready for homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, 
            Heller S
  TITLE     Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in
            vitro-generated inner ear sensory epithelia cells and enable otic 
            lineage selection and Cre-recombination.
  JOURNAL   Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013.
  PUBMED    30179592
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013."
COMMENT     Sequence Label: pmTHC_PGKneoLox2DTA.2
FEATURES             Location/Qualifiers
     source          1..11228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             734..1111
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             1118..1825
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             1835..1891
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1892..2914
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1892..2914
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2927..2983
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2984..4012
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4025..4960
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4073..4966
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5054..5073)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5119..5174
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5173..5192)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5535..5551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5559..5575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5598..5614
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5598..5614
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    5628..5661
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5691..6190
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6204..7007
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6267..6286)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6877..6896
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7045..7269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7328..7361
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7440..7939
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7966..8538
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          8615..8680
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    8754..8978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9040..9060)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9040..9058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9079..9095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9127..9133,9134..9151,9152..9157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9481..10069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9561..9580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10240..11031,11032..11100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10863..10882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11101..11205)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcctag gagatctacg cgtcaattga tgcatcccgg gtttattcat
      721 gaggccggcc accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa
      781 gaaggcggtg actaaggcgc agaagaaaga cggcaagaag cgcaagcgca gccgcaagga
      841 gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc
      901 gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg
      961 tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca
     1021 gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac
     1081 taaggccgtc accaagtaca ccagcgctaa atccaccatg gtgagcaagg gcgaggagac
     1141 cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa
     1201 tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg gcaccaacac
     1261 catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca ttctgaccac
     1321 cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc ccaactactt
     1381 caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg aggacaaggg
     1441 catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct acgagataca
     1501 cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga ccaccggctg
     1561 ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca
     1621 caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca tctacagggc
     1681 caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg agatcctgaa
     1741 ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc gcaactccac
     1801 cgacggcatg gacgagctgt acaagggaag cggagctact aacttcagcc tgctgaagca
     1861 ggctggagac gtggaggaga accctggacc tatgaaaaag cctgaactca ccgcgacgtc
     1921 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     1981 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     2041 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     2101 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     2161 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     2221 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     2281 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     2341 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     2401 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     2461 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     2521 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     2581 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     2641 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     2701 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     2761 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     2821 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     2881 ccgacgcccc agcactcgtc cgagggcaaa ggaacgcggc tccggcgcaa caaatttctc
     2941 tctcctcaaa caagcaggcg atgttgaaga aaatcccggg cccatgtcca atttactgac
     3001 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     3061 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     3121 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     3181 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     3241 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     3301 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     3361 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     3421 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     3481 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     3541 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     3601 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     3661 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     3721 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     3781 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     3841 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     3901 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     3961 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc
     4021 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     4081 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     4141 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     4201 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     4261 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     4321 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     4381 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     4441 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     4501 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     4561 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     4621 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     4681 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     4741 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     4801 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     4861 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     4921 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatagc ggccgcactc
     4981 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     5041 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     5101 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     5161 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     5221 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     5281 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     5341 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5401 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5461 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     5521 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5581 cacaaaaact aggccgctct agaactagtg gatccggaac ccttaatata acttcgtata
     5641 atgtatgcta tacgaagtta ttaggtccct cgacctgcag gaattctacc gggtagggga
     5701 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5761 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5821 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5881 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5941 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     6001 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     6061 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     6121 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     6181 gggcctttcg acctgcagcc aatatgggat cggccattga acaagatgga ttgcacgcag
     6241 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6301 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6361 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6421 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6481 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6541 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6601 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6661 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6721 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
     6781 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6841 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6901 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6961 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattct
     7021 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7081 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7141 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7201 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7261 gctctatggc ttctgaggcg gaaagaacca gctggggctc gaatcaagct gatccggaac
     7321 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtccct cgacctgcag
     7381 cccaagctag cttatcgata ccgtcgacgg tatcgataag cttgatatcg aattctaccg
     7441 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     7501 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     7561 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     7621 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7681 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7741 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7801 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7861 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7921 ctcatctccg ggcctttcga cctgcaggtc ctcgccatgg atcctgatga tgttgttgat
     7981 tcttctaaat cttttgtgat ggaaaacttt tcttcgtacc acgggactaa acctggttat
     8041 gtagattcca ttcaaaaagg tatacaaaag ccaaaatctg gtacacaagg aaattatgac
     8101 gatgattgga aagggtttta tagtaccgac aataaatacg acgctgcggg atactctgta
     8161 gataatgaaa acccgctctc tggaaaagct ggaggcgtgg tcaaagtgac gtatccagga
     8221 ctgacgaagg ttctcgcact aaaagtggat aatgccgaaa ctattaagaa agagttaggt
     8281 ttaagtctca ctgaaccgtt gatggagcaa gtcggaacgg aagagtttat caaaaggttc
     8341 ggtgatggtg cttcgcgtgt agtgctcagc cttcccttcg ctgaggggag ttctagcgtt
     8401 gaatatatta ataactggga acaggcgaaa gcgttaagcg tagaacttga gattaatttt
     8461 gaaacccgtg gaaaacgtgg ccaagatgcg atgtatgagt atatggctca agcctgtgca
     8521 ggaaatcgtg tcaggcgatc tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8581 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8641 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8701 tgaatgggag cagtggtgga atgcagatcc tagagctcgc tgatcagcct cgactgtgcc
     8761 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8821 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     8881 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8941 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     9001 ctggggctcg agggggggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     9061 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11221 agtgccac
//
