LOCUS       sequence-22221-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J
  TITLE     Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of
            the TGFbeta pathway.
  JOURNAL   Cell. 2006 Jun 2. 125(5):929-41.
  PUBMED    16751102
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 2. 125(5):929-41."
COMMENT     Sequence Label: pRetroSuper-GFP Smad4
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(73..89)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(74..90)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(104..520)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(120..136)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(152..174)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 agatccccgg tgtgcagttg gaatgtattc aagagataca ttccaactgc acaccttttt
       61 ggaaaagctt atcgataccg tcgacctcga gagatctaat tccggcgcct agagaaggag
      121 tgagggctgg ataaagggag gatcgaggcg gggtcgaacg aggaggttca agggggagag
      181 acggggcgga tggaggaaga ggaggcggag gcttagggtg tacaaagggc ttgacccagg
      241 gaggggggtc aaaagccaag gcttcccagg tcacgatgta ggggacctgg tctgggtgtc
      301 catgcgggcc aggtgaaaag accttgatct taacctgggt gatgaggtct cggttaaagg
      361 tgccgtctcg cggccatccg acgttaaagg ttggccattc tgcagagcag aaggtaaccc
      421 aacgtctctt cttgacatct accgactggt tgtgagcgat ccgctcgaca tctttccagt
      481 gacctaaggt caaacttaag ggagtggtaa cagtctggcc ctaattttca gacaaataca
      541 gaaacacagt cagacagaga caacacagaa cgatgctgca gcgctgcagc agacaagacg
      601 cgcggcttcg gttccaaacc gaaagcaaaa attcagacgg aggcgggaac tgttttaggt
      661 tctcgtctcc taccagaacc acatatcctg acggggtcgg attccacatc gactcccttc
      721 ctcaggtcgg gccacaaaaa cggcccccaa agtccctggg acgtctccca gggttgcggc
      781 cgggtgttca gaactcgtca gttccaccac gggtccgcca gatacagagc tagttagcta
      841 actagtacag acgcaggcgc aaacattaga tgccggcaca aacacgccca aagaccgaga
      901 cagacacgaa acgaccgctg gccagc
//
