LOCUS       sequence-22242-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pET15-UbE2D2
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(68..76,77..91)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
       61 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagatctg cagatcaaac
      121 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatggctc tgaagagaat
      181 ccacaaggaa ttgaatgatc tggcacggga ccctccagca cagtgttcag caggtcctgt
      241 tggagatgat atgttccatt ggcaagctac aataatgggg ccaaatgaca gtccctatca
      301 gggtggagta tttttcttga caattcattt cccaacagat taccccttca aaccacctaa
      361 ggttgcattt acaacaagaa tttatcatcc aaatattaac agtaatggca gcatttgtct
      421 tgatattcta cgatcacagt ggtctccagc actaactatt tcaaaagtac tcttgtccat
      481 ctgttctctg ttgtgtgatc ccaatccaga tgatccttta gtacctgaga ttgctcggat
      541 ctacaaaaca gatagagaaa agtacaacag aatagctcgg gaatggactc agaagtatgc
      601 gatgtaactc gagggaagg
//
