LOCUS       sequence-22248-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2G1
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..94
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(98..106,107..121)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    149..173
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatata gcggggttct catcatcatc atcatcatgg
       61 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      121 ggatccgagc tcgagatctg cagatcaaac aagtttgtac aaaaaagttg gcatgacgga
      181 gctacagtcg gcactgctac tgcgaagaca gctggcagaa ctcaacaaaa atccagtgga
      241 aggcttttct gcaggtttaa tagatgacaa tgatctctac cgatgggaag tccttattat
      301 tggccctcca gatacacttt atgaaggtgg tgtttttaag gctcatctta ctttcccaag
      361 aagatatatc ccctccgacc tcctaaaatg aaattcatta cagaaatctg gcacccaaat
      421 gttgataaaa atggtgatgt gtgcatttct attcttcatg agcctgggga agataagtat
      481 ggttatgaaa agccagagga acgctggctc cctatccaca ctgtggaaac catcatgatt
      541 agtgtcattt ctatgctggc agaccctaat ggagactcac ctgctaatgt tgatgctgcg
      601 aaagaatgga gggaagatag aaatggagaa tttaaaagaa aagttgcccg ctgt
//
