LOCUS       sequence-22252-e         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2L6
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..91
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(95..103,104..118)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    146..170
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttactttaag aaggagatat acatatagcg gggttctcat catcatcatc atcatggtat
       61 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga
      121 tccgagctcg agatctgcag atcaaacaag tttgtacaaa aaagttggca tgatggcgag
      181 catgcgagtg gtgaaggagc tggaggatct tcagaagaag cctcccccat acctgcggaa
      241 cctgtccagc gatgatgcca atgtcctggt gtggcacgct ctcctcctac ccgaccaacc
      301 tccctaccac ctgaaagcct tcaacctgcg catcagcttc ccgccggagt atccgttcaa
      361 gcctcccatg atcaaattca caaccaagat ctaccacccc aacgtggacg agaacggaca
      421 gatttgcctg cccatcatca gcagtgagaa ctggaagcct tgcaccaaga cttgccaagt
      481 cctggaggcc ctcaatgtgc tggtgaatag accgaatatc agggagcccc tgcggatgga
      541 cctcgctgac ctgctgacac agaatccgga gctgttcaga aagaatgccg aagagttcac
      601 cctccgattc ggagtggacc ggccctcgta cccaactttc ttgt
//
