LOCUS       sequence-22273-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, 
            Young JA, Callaway EM
  TITLE     Monosynaptic restriction of transsynaptic tracing from single, 
            genetically targeted neurons.
  JOURNAL   Neuron. 2007 Mar 1. 53(5):639-47.
  PUBMED    17329205
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2007 Mar 1. 53(5):639-47."
COMMENT     Sequence Label: pCAG-DsRed2
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    42..94
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(288..308)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ttgtggcggc cgcctgggcc ggtgaccgta ccgggccccc cctcgagctc aagcttcgaa
       61 ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg gcctcctccg
      121 agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc
      181 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga
      241 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
      301 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc
      361 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      421 cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca
      481 tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg
      541 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc
      601 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atg
//
