LOCUS       sequence-22284-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG25QDProGFP p306
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             187..897
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAH
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(214..242)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     837..857
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(923..941)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ctagactacg ccatggacta caaggacgac gatgacaagc tggcgaccct ggaaaagctg
       61 atgaaggcct tcgagtccct caaaagcttc caacagcagc aacagcaaca acagcagcaa
      121 cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaactgca gcccggggga
      181 tccctgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      241 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      301 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      361 cttgtcacta ctttgacttg gggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
      421 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      481 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      541 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      601 aaattggaat acaactatat ttcacacaat gtatacatca ctgcagacaa acaaaagaat
      661 ggaatcaaag ctcatttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      721 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      781 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      841 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaatag
      901 ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctccccca catccgctct
      961 aaccgaaaag gaaggag
//
