LOCUS       sequence-22286-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG72QDProGFP p306
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..23
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(340..368)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actacaagga cgacgatgac aagctgggcg accctggaaa agctaatgaa ggccttcgag
       61 tccctcaaaa gcttccaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      121 caacagcaac aacagcagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcagc agcaacaaca gcagcaacag caacaacaac agcagcaaca gcaacaacag
      241 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca actgcagccc
      301 gggggatccc tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      361 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      421 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      481 ccaacacttg tcactacttt gacttggggt gttcaatgct tttcaagata cccagatcat
      541 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      601 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      661 acccttgtta atagaatcga gttaaaaggt attgat
//
