LOCUS       sequence-222895-        9462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PinCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0058	PinCas13 (B02) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(31..54,55..90,91..114)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             115..408
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(3837..3855)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3851..3898
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4073..4078,4079..4095,4096..4101))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4128..4146)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4214..4318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4319..4387,4388..5179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4537..4556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5350..5938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5839..5858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6092..6109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6124..6263
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6189..6208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6349..6371
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6365..6556)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7868..8950)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8912..8931
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8951..9028)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9234..9253
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9337..9356
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9337..9355
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9356..9380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9395..9417
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9436..9453
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
       61 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
      121 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
      181 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
      241 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
      301 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
      361 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc tatggaggac
      421 gataagaaaa ccacagatag catcagatac gagctgaagg acaagcactt ctgggccgcc
      481 tttctgaatc tggccaggca caacgtgtat atcaccgtga atcacatcaa caagatcctg
      541 gaggagggcg agatcaaccg cgatggctac gagacaaccc tgaagaatac atggaacgag
      601 atcaaggata tcaataagaa ggaccgcctg tccaagctga tcatcaagca cttccccttt
      661 ctggaggccg ccacctaccg gctgaaccct acagatacca caaagcagaa ggaggagaag
      721 caggcagagg cacagtccct ggagtctctg cggaagagct tcttcgtgtt catctacaag
      781 ctgagggacc tgcgcaatca ctactctcac tataagcaca gcaagagcct ggagcggccc
      841 aagttcgagg agggcctgct ggagaagatg tataacatct ttaacgccag catcaggctg
      901 gtgaaggagg attaccagta taataaggac atcaaccctg acgaggattt caagcacctg
      961 gatcgcaccg aggaggagtt caactactac ttcaccaagg acaatgaggg caacatcaca
     1021 gagagcggcc tgctgttctt cgtgagcctg ttcctggaga agaaggatgc catctggatg
     1081 cagcagaagc tgcgcggctt caaggacaat cgggagaaca agaagaagat gaccaacgag
     1141 gtgttttgca ggagccgcat gctgctgcct aagctgcgcc tgcagtccac ccagacacag
     1201 gattggattc tgctggacat gctgaatgag ctgatccggt gtccaaagtc cctgtacgag
     1261 cggctgagag aggaggaccg cgagaagttc cgggtgccca tcgagatcgc cgacgaggat
     1321 tatgacgccg agcaggagcc ttttaagaac accctggtga gacaccagga taggttccca
     1381 tactttgccc tgagatactt cgactataat gagatcttca ccaacctgag gtttcagatc
     1441 gatctgggca cataccactt ttctatctat aagaagcaga tcggcgacta caaggagagc
     1501 caccacctga cccacaagct gtatggcttc gagagaatcc aggagtttac aaagcagaac
     1561 cgccccgatg agtggcggaa gttcgtgaaa accttcaact cttttgagac aagcaaggag
     1621 ccatacatcc ccgagacaac acctcactat cacctggaga accagaagat cggcatcagg
     1681 tttcgcaacg ataatgacaa gatctggccc agcctgaaaa ccaacagcga gaagaacgag
     1741 aagtctaagt acaagctgga caagagcttc caggccgagg cctttctgag cgtgcacgag
     1801 ctgctgccaa tgatgttcta ctatctgctg ctgaaaaccg agaacacaga taacgacaat
     1861 gagatcgaga caaagaagaa ggagaacaag aatgataagc aggagaagca caagatcgag
     1921 gagatcatcg agaacaagat caccgagatc tacgccctgt atgacacatt cgccaatggc
     1981 gagatcaagt ccatcgatga gctggaggag tactgcaagg gcaaggacat cgagatcggc
     2041 cacctgccca agcagatgat cgccatcctg aaggacgagc acaaagtgat ggccaccgag
     2101 gccgagagaa agcaggagga gatgctggtg gatgtgcaga agagcctgga gtccctggac
     2161 aatcagatca acgaggagat cgagaatgtg gagagaaaga acagctccct gaagtccggc
     2221 aagatcgcct cttggctggt gaacgatatg atgaggttcc agcctgtgca gaaggacaac
     2281 gagggcaagc cactgaacaa ttccaaggcc aattctaccg agtatcagct gctgcagcgc
     2341 acactggcct tctttggctc cgagcacgag cggctggccc cttacttcaa gcagaccaag
     2401 ctgatcgagt ctagcaatcc ccaccctttt ctgaaggata cagagtggga gaagtgtaac
     2461 aatatcctga gcttctacag gtcctatctg gaggccaaga agaactttct ggagtctctg
     2521 aagccagagg actgggagaa gaatcagtat ttcctgaagc tgaaggagcc taagaccaag
     2581 ccaaagacac tggtgcaggg ctggaagaat ggcttcaacc tgccacgcgg catctttacc
     2641 gagcccatca gaaagtggtt tatgaagcac agggagaaca tcacagtggc agagctgaag
     2701 agggtgggcc tggtggccaa agtgatcccc ctgttcttta gcgaggagta caaggattcc
     2761 gtgcagcctt tctacaatta tcactttaac gtgggcaaca tcaataagcc agacgagaag
     2821 aatttcctga actgcgagga gcggagagag ctgctgcgga agaagaagga tgagtttaag
     2881 aagatgaccg acaaggagaa ggaggagaac ccctcttatc tggagttcaa gagctggaat
     2941 aagtttgagc gggagctgag actggtgagg aaccaggata tcgtgacatg gctgctgtgc
     3001 atggagctgt tcaacaagaa gaagatcaag gagctgaatg tggagaagat ctacctgaag
     3061 aacatcaata ccaacaccac aaagaaggag aagaatacag aggagaagaa cggcgaggag
     3121 aagaatatca aggagaagaa caatatcctg aacagaatca tgcccatgag gctgcctatc
     3181 aaggtgtatg gcagagagaa tttcagcaag aacaagaaga agaagatcag gcgcaacacc
     3241 ttctttaccg tgtatatcga ggagaagggc accaagctgc tgaagcaggg caacttcaag
     3301 gccctggaga gggaccggag actgggaggc ctgttcagct tcgtgaaaac cccctctaag
     3361 gccgagtcta agagcaacac aatctctaag ctgcgcgtgg agtacgagct gggcgagtat
     3421 cagaaggccc ggatcgagat catcaaggat atgctggccc tggagaaaac cctgatcgac
     3481 aagtataata gcctggatac cgacaatttc aacaagatgc tgacagattg gctggagctg
     3541 aagggcgagc ctgacaaggc cagcttccag aacgatgtgg acctgctgat cgccgtgcgg
     3601 aacgcctttt cccacaatca gtacccaatg cggaatagaa tcgccttcgc caatatcaac
     3661 cccttttccc tgtcctctgc caacacctct gaggagaagg gcctgggcat cgccaatcag
     3721 ctgaaggaca agacccacaa gacaatcgag aagatcatcg agatcgagaa gcccatcgag
     3781 acaaaggagt aaggccgcac tcgaggcccg aaaggaagct gagttggctg ctgccaccgc
     3841 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     3901 gaaaggagga actatatccg gatatcccgc aagaggcccg gcagtaccgg cataaccaag
     3961 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
     4021 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc
     4081 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
     4141 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4561 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4861 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcacg
     5041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5461 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6121 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
     6181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     6241 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     6301 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6361 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     6421 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     6481 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     6541 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     6601 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     6661 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     6721 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     6781 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     6841 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     6901 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     6961 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     7021 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     7081 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     7141 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     7201 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     7261 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     7321 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     7381 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     7441 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     7501 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     7561 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     7621 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     7681 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     7741 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     7801 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     7981 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     8041 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     8101 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     8161 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     8221 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     8281 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     8341 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     8401 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     8461 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     8521 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     8581 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     8641 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     8701 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     8761 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     8821 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     8881 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     8941 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     9001 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     9061 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     9121 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     9181 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     9241 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     9301 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     9361 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     9421 accatgggca gcagccatca tcatcatcat cacagcagcg gc
//
