LOCUS       sequence-22290-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Tee AR, Manning BD, Roux PP, Cantley LC, Blenis J
  TITLE     Tuberous sclerosis complex gene products, Tuberin and Hamartin, 
            control mTOR signaling by acting as a GTPase-activating protein 
            complex toward Rheb.
  JOURNAL   Curr Biol. 2003 Aug 5. 13(15):1259-68.
  PUBMED    12906785
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Aug 5. 13(15):1259-68."
COMMENT     Sequence Label: pRK7-Rheb
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(583..606)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(676..695)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(708..726)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(709..726)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gttgggccat ggcggccatc gattgaattc tcacatcacc gagcatgaag acttgccttg
       61 tgaagctgcc ccgtccattt tttctgcctc caaaattatc cttcgaaaaa catccacagc
      121 agtctgattt tctttagcag aagattccaa aaaagctgca ttccaagatt ctgccaaagc
      181 tttcccttct tcataactga tcaccctttc catatgcagg tctttcttat tcccaaccaa
      241 cataataggt atttgtactt tccccaccat atccaacaat ttgccatgga taactttaat
      301 cacttcaaaa cttttgattg atgtaacaga atacacaaga atatagccat taatatctat
      361 ggagtatgtc tgaggaaaga tagaatattc atcttgcccg gctgtgtcta caagttgaag
      421 atgatattct tgtccattta ctgtgatcaa ctttgtaaaa gtgttttcta tggttggatc
      481 gtaggagtcc acaaattggc cttcaacaaa ttgaatcgtc aatgaggatt tccccacaga
      541 ccggtagccc aggatcgcga tcttccggga cttggactgc ggtttgtcat catcgtcctt
      601 atagtccatg gtggatcctc tagagtcgac ctgcagaagc ttagaaccga ggtgcagttg
      661 gacctgggag tggacacctg tggagagaaa ggcaaagtgg atgttattct atagtgtcac
      721 ctaaatcgta tgtgtatg
//
