LOCUS       sequence-222907-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Pin2Cas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0065	Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     284..303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     387..406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        387..405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    406..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             445..467
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             513..530
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(543..566,567..602,603..626)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             627..920
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(4331..4349)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4345..4392
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4567..4572,4573..4589,4590..4595))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4622..4640)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4708..4812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4813..4881,4882..5673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5031..5050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5844..6432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6333..6352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6586..6603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6618..6757
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6683..6702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6843..6865
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6859..7050)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8362..9444)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9406..9425
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      481 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta
      541 gctggagcca tccgcagttt gaaaaaggtg gtggtagcgg tggtggttca ggtggtagtg
      601 catggtcaca ccctcagttt gagaaaatgt cggactcaga agtcaatcaa gaagctaagc
      661 cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg tccgatggat
      721 cttcagagat cttcttcaag atcaaaaaga ccactccttt aagaaggctg atggaagcgt
      781 tcgctaaaag acagggtaag gaaatggact ccttaagatt cttgtacgac ggtattagaa
      841 tccaagctga tcagacccct gaagatttgg acatggagga taacgatatt attgaggctc
      901 acagagaaca gattggtgga tctatggaag atgacaagaa aaccaccgac agcatccgct
      961 acgagctgaa ggacaagcac ttctgggccg cctttctgaa cctggccaga cacaacgtgt
     1021 acatcaccgt gaaccacatc aacaagatcc tggaagagga cgagatcaac cgcgacggct
     1081 acgagaacac cctggaaaac agctggaatg agatcaagga cattaacaag aaggaccggc
     1141 tgagcaagct gatcatcaag cactttccat ttctggaagc caccacctac cggcagaacc
     1201 ccaccgatac caccaagcag aaagaggaaa agcaggccga ggctcagagc ctggaaagcc
     1261 tgaagaaatc cttcttcgtg ttcatctaca agctgcggga cctgcggaac cactacagcc
     1321 actacaagca cagcaagtcc ctggaacggc ccaagttcga agaggacctg cagaacaaga
     1381 tgtacaacat cttcgacgtg tccatccagt tcgtgaaaga ggactataag cacaacaccg
     1441 acatcaaccc taagaaggac ttcaagcacc tggaccggaa gcggaagggc aagttccact
     1501 actccttcgc cgacaacgag ggcaacatca cagagagcgg cctgctgttc tttgtgtccc
     1561 tgttcctgga aaagaaagac gccatctggg tgcagaagaa gctggaaggc ttcaagtgca
     1621 gcaacaagag ctaccagaaa atgaccaacg aggtgttctg tcggagccgg atgctgctgt
     1681 ccaagctgag actggaaagc acccagacac aggactggat cctgctggac atgctgaacg
     1741 agctgatcag atgccccaag agcctgtacg agagactgca gggcgtgaac cggaagaagt
     1801 tctacgtcag cttcgacccc gccgacgagg attacgatgc cgagcaagag cccttcaaga
     1861 ataccctcgt gcggcaccag gacagattcc cttacttcgc cctgcggtac ttcgactaca
     1921 atgaggtgtt cgccaacctg cggttccaga tcgacctggg cacctaccac ttcagcatct
     1981 ataagaagct gatcggcggc cagaaagaag atcggcacct gacacacaag ctgtacggct
     2041 tcgagcggat ccaagagttc gacaagcaga acagacccga cgagtggaag gccatcgtga
     2101 aggacagcga cacctttaag aaaaaagaag aaaaagagga agagaagccc tacatcagcg
     2161 agacaacccc tcactaccac ctcgaaaaca agaagattgg gatcgccttc aaaaaccaca
     2221 acatctggcc cagcacacag accgagctga ccaacaacaa gcgcaagaag tacaatctgg
     2281 ggacctctat caaggctgag gccttcctgt ctgtgcacga gctgctgcct atgatgttct
     2341 actacctgct gctgaaaacc gagaatacca agaacgacaa caaagtcggc gggaagaaag
     2401 agactaagaa gcagggcaag cacaagatcg aggccatcat cgagagcaag attaaggaca
     2461 tctacgccct gtacgacgcc ttcgccaacg gcgagatcaa tagcgaggat gagctgaaag
     2521 agtacctgaa gggcaaagac atcaagatcg tgcatctgcc gaagcagatg atcgccatcc
     2581 tgaagaacga gcacaaggac atggccgaga aggccgaagc caagcaagag aagatgaagc
     2641 tggccaccga gaacagactg aaaaccctgg acaagcagct caaggggaag atccagaacg
     2701 gcaagcggta caacagcgcc cctaagtctg gcgagattgc ctcctggctg gtcaacgaca
     2761 tgatgcggtt tcagcccgtg cagaaggacg agaacggcga gtccctgaac aacagcaagg
     2821 ccaacagcac cgagtaccag ctgctgcaga gaaccctggc cttctttgga agcgagcacg
     2881 aaagactggc cccttacttt aagcagacca agctgattga gagcagcaac cctcatcctt
     2941 ttctgaacga caccgagtgg gagaagtgct ccaacatcct gagcttctac cggtcctatc
     3001 tgaaggcccg gaagaacttc ctggaatccc tgaagcctga ggactgggag aagaaccagt
     3061 acttcctgat gctcaaagag cccaagacca accgggaaac cctggtgcaa ggctggaaga
     3121 acggcttcaa cctgcctcgg ggcttcttca ccgagcctat ccggaagtgg ttcatggaac
     3181 actggaagtc catcaaggtg gacgacctga agcgcgttgg cctggtggct aaagtgaccc
     3241 ctctgttctt cagcgagaag tataaggact ccgtgcagcc cttctacaac taccccttca
     3301 acgtgggcga cgtgaacaag cccaaagaag aggacttcct gcaccgcgag gaacggatcg
     3361 agctgtggga caagaagaag gacaaattca agggctacaa ggccaagaaa aagttcaaag
     3421 agatgaccga caaagagaaa gaagaacacc ggtcctacct tgagttccag agctggaaca
     3481 agttcgagcg cgagctgagg ctcgtgcgca atcaggatat cgtgacctgg ctgctgtgca
     3541 ccgaactgat cgacaagctg aagatcgacg aactgaacat caaagagctg aagaagctcc
     3601 ggctgaagga tatcaatacc gacaccgcca agaaagaaaa gaacaacatt ctgaaccgcg
     3661 tgatgcccat ggaactgccc gtgaccgtgt acaaagtgaa caaaggcggc tacatcatca
     3721 agaacaagcc tctgcacacc atctatatca aagaggccga gacaaagctg ctgaagcagg
     3781 gaaacttcaa ggccctggtc aaggacagac ggctgaatgg cctgttcagc ttcgtgaaaa
     3841 cccctagcga ggccgagtcc gagagcaacc ctatctctaa actgcgcgtg gaatacgagc
     3901 tgggcaagta ccagaacgcc cggctggata tcatcgagga tatgctggcc ctcgaaaaga
     3961 agctcatcga caagtacaac tccctggaca ccgacaactt ccacaacatg ctgaccggct
     4021 ggctggaact gaaaggcgag gccaaaaagg cccggtttca gaacgacgtg aaactgctga
     4081 ccgccgtgcg gaatgccttc agccacaatc agtaccctat gtacgacgag aacctgttcg
     4141 gcaacatcga gcggttcagc ctgtcctcca gcaacatcat tgagtccaaa ggcctggata
     4201 tcgccgccaa actcaaagaa gaagtgtcca aggccgccaa aaagatccaa aacgaggaag
     4261 ataacaagaa agagaaagaa acctaaggcc gcactcgagg cccgaaagga agctgagttg
     4321 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     4381 aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg cccggcagta
     4441 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
     4501 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
     4561 taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
     4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5041 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5341 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5521 gagcgtgggt cacgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6541 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6601 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     6661 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     6721 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     6781 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6841 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     6901 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     6961 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     7021 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     7081 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     7141 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     7201 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     7261 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     7321 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     7381 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     7441 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     7501 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     7561 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     7621 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
     7681 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     7741 acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg
     7801 gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt aagagccgcg
     7861 agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     7921 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     7981 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata
     8041 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     8101 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     8161 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     8221 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     8281 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     8341 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     8461 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     8521 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     8581 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     8641 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     8701 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     8761 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     8821 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     8881 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     8941 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     9001 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     9061 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     9121 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     9181 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     9241 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     9301 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     9361 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     9421 atcgtataac gttactggtt tcac
//
