LOCUS       sequence-222913-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PauCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0063	PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(138..161,162..197,198..221)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             222..515
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(3938..3956)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3952..3999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4174..4179,4180..4196,4197..4202))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4229..4247)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4315..4419
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4420..4488,4489..5280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4638..4657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5451..6039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5940..5959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6193..6210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6225..6364
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6290..6309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6450..6472
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6466..6657)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7969..9051)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9013..9032
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9052..9129)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9335..9354
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     join(9438..9456,1)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9438..9456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      121 gcagccatat ggctagctgg agccatccgc agtttgaaaa aggtggtggt agcggtggtg
      181 gttcaggtgg tagtgcatgg tcacaccctc agtttgagaa aatgtcggac tcagaagtca
      241 atcaagaagc taagccagag gtcaagccag aagtcaagcc tgagactcac atcaatttaa
      301 aggtgtccga tggatcttca gagatcttct tcaagatcaa aaagaccact cctttaagaa
      361 ggctgatgga agcgttcgct aaaagacagg gtaaggaaat ggactcctta agattcttgt
      421 acgacggtat tagaatccaa gctgatcaga cccctgaaga tttggacatg gaggataacg
      481 atattattga ggctcacaga gaacagattg gtggatctat ggaggacgat aagaaaacca
      541 caggcagcat ctcctacgag ctgaaggaca agcacttctg ggccgccttt ctgaacctgg
      601 cccgccacaa cgtgtatatc accatcaacc acatcaataa gctgctggag atccgggaga
      661 tcgacaacga tgagaaggtg ctggacatca agaccctgtg gcagaagggc aacaaggatc
      721 tgaatcagaa ggccaggctg cgcgagctga tgacaaagca cttccccttc ctggagacag
      781 ccatctacac aaagaacaag gaggataaga aggaggtgaa gcaggagaag caggccgagg
      841 cccagtctct ggagagcctg aaggactgcc tgttcctgtt tctggataag ctgcaggagg
      901 cccgcaatta ctatagccac tacaagtatt ccgagttctc taaggagcca gagtttgagg
      961 agggcctgct ggagaagatg tataacatct ttggcaacaa tatccagctg gtcatcaacg
     1021 actatcagca caacaaggat atcaatcccg acgaggattt caagcacctg gaccggaagg
     1081 gccagttcaa gtactccttt gccgataacg agggcaatat caccgagtcc ggcctgctgt
     1141 tcttcgtgag cctgttcctg gagaagaagg acgccatctg gatgcagcag aagctgaacg
     1201 gctttaagga taacctggag aacaagaaga agatgacaca cgaggtgttc tgccggagca
     1261 gaatcctgat gcccaagctg cgcctggaga gcacccagac acaggactgg attctgctgg
     1321 acatgctgaa tgagctgatc cggtgtccta agtccctgta cgagagactg cagggcgacg
     1381 atagggagaa gttcaaggtg ccatttgacc ccgccgacga ggattacaac gccgagcagg
     1441 agccttttaa gaataccctg atcagacacc aggacaggtt cccatacttt gtgctgagat
     1501 acttcgatta caacgagatc ttcaagaatc tgaggtttca gatcgacctg ggcacctacc
     1561 acttctctat ctataagaag ctgatcggcg gccagaagga ggatcgccac ctgacacaca
     1621 agctgtacgg cttcgagcgc atccaggagt ttgccaagca gaaccggcct gacgagtgga
     1681 aggccatcgt gaaggacctg gatacctacg agacaagcaa caagcggtat atctccgaga
     1741 caacacctca ctatcacctg gagaatcaga agatcggcat caggtttcgc aacggcaata
     1801 aggagatctg gcccagcctg aaaaccaacg acgagaacaa tgagaagtct aagtacaagc
     1861 tggataagca gtatcaggcc gaggcctttc tgtccgtgca cgagctgctg cccatgatgt
     1921 tctactatct gctgctgaag aaggagaagc ctaacaatga cgagatcaat gcctctatcg
     1981 tggagggctt catcaagcgg gagatcagaa acatcttcaa gctgtacgac gcctttgcca
     2041 atggcgagat caacaatatc gacgatctgg agaagtattg cgccgataag ggcatcccca
     2101 agagacacct gcctaagcag atggtggcca tcctgtacga cgagcacaag gatatggtga
     2161 aggaggccaa gaggaagcag aaggagatgg tgaaggatac caagaagctg ctggccaccc
     2221 tggagaagca gacacagaag gagaaggagg acgatggcag aaacgtgaag ctgctgaaga
     2281 gcggcgagat cgccagatgg ctggtgaatg acatgatgag gttccagccc gtgcagaagg
     2341 ataacgaggg caagcctctg aacaatagca aggccaattc caccgagtac cagatgctgc
     2401 agcggagcct ggccctgtat aacaatgagg agaagcccac aaggtatttt cgccaggtga
     2461 acctgatcga gagcaacaat ccccaccctt tcctgaagtg gaccaagtgg gaggagtgta
     2521 acaacatcct gaccttttac tactcttacc tgacaaagaa gatcgagttc ctgaacaagc
     2581 tgaagccaga ggactggaag aagaatcagt attttctgaa gctgatggag cccaagacca
     2641 acagagagac actggtgcag ggctggaaga acggctttaa tctgcctagg ggcatcttca
     2701 cagagccaat ccgcgagtgg ttcaagcggc accagaacaa tagcaaggag tacgagaagg
     2761 tggaggccct ggacagagtg ggcctggtga ccaaagtgat ccccctgttc tttaaggagg
     2821 agtacttcaa ggataaggag gagaacttca aggaggacac acagaaggag atcaacgatt
     2881 gcgtgcagcc attctacaat tttccctata acgtgggcaa tatccacaag cctaaggaga
     2941 aggactttct gcaccgggag gagagaatcg agctgtggga caagaagaag gataagttca
     3001 agggctacaa ggagaagatc aagagcaaga agctgaccga gaaggacaag gaggagttca
     3061 ggtcttatct ggagtttcag agctggaaca agttcgagag ggagctgcgc ctggtgcgga
     3121 atcaggatat cgtgacatgg ctgctgtgca aggagctgat cgacaagctg aagatcgatg
     3181 agctgaacat cgaggagctg aagaagctga gactgaacaa tatcgacacc gatacagcca
     3241 agaaggagaa gaacaatatc ctgaatagag tgatgcctat ggagctgcca gtgaccgtgt
     3301 acgagatcga cgattcccac aagatcgtga aggacaagcc actgcacacc atctatatca
     3361 aggaggccga gacaaagctg ctgaagcagg gcaacttcaa ggccctggtg aaggatcgga
     3421 gactgaatgg cctgttcagc ttcgtgaaaa ccaacagcga ggccgagagc aagcgcaatc
     3481 ctatctccaa gctgcgggtg gagtacgagc tgggcgagta tcaggaggcc cggatcgaga
     3541 tcatccagga catgctggcc ctggaggaga agctgatcaa caagtacaag gatctgccaa
     3601 caaacaagtt ttccgagatg ctgaacagct ggctggaggg caaggacgag gccgataagg
     3661 cccgctttca gaatgacgtg gatttcctga tcgccgtgcg gaacgccttc tcccacaatc
     3721 agtacccaat gcacaacaag atcgagtttg ccaatatcaa gcccttcagc ctgtataccg
     3781 ccaacaattc cgaggagaag ggcctgggca tcgccaacca gctgaaggac aagaccaagg
     3841 agacaacaga taagatcaag aagatcgaga agcccatcga gacaaaggag taaggccgca
     3901 ctcgaggccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     3961 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     4021 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     4081 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     4141 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     4201 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4261 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4621 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     4681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     4981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5101 tttattgctg ataaatctgg agccggtgag cgtgggtcac gcggtatcat tgcagcactg
     5161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5581 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6181 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     6241 ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct
     6301 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     6361 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6421 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6481 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     6541 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     6601 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     6661 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     6721 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     6781 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     6841 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     6901 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     6961 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     7021 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     7081 tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct
     7141 gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
     7201 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
     7261 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
     7321 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc
     7381 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg
     7441 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca
     7501 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     7561 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
     7621 agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg
     7681 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg
     7741 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc
     7801 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
     7861 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     7921 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     7981 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8041 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     8101 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     8161 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     8221 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     8281 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     8341 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     8401 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     8461 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     8521 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     8581 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     8641 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     8701 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     8761 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     8821 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     8881 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     8941 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     9001 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     9061 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     9121 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     9181 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     9241 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     9301 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     9361 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     9421 tctcgatccc gcgaaattaa tacgactcac tatagg
//
