LOCUS       sequence-222929-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX313-Firefly luciferase
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..133)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caaagctcat ctttccaaat tttgtaatcc agaggttgat tgtcgactta acgcgttgat
       61 tgtcgactta accggtacta gttcattact acgtagaatc gagaccgagg agagggttag
      121 ggataggctt accgatatca accactttgt acaagaaagt tggcaacacg gcgatctttc
      181 cgcccttctt ggcctttatg aggatctctc tgatttttct tgcgtcgagt tttccggtaa
      241 gacctttcgg tacttcgtcc acaaacacaa ctcctccgcg caactttttc gcggttgtta
      301 cttgactggc gacgtaatcc acgatctctt tttccgtcat cgtctttccg tgctccaaaa
      361 caacaacggc ggcgggaagt tcaccggcgt catcgtcggg aagacctgcg acacctgcgt
      421 cgaagatgtt ggggtgttgg agcaagatgg attccaattc agcgggagcc acctgatagc
      481 ctttgtactt aatcagagac ttcaggcggt caacgatgaa gaagtgttcg tcttcgtccc
      541 agtaagctat gtctccagaa tgtagccatc catccttgtc aatcaaggcg ttggtcgctt
      601 ccggattgtt tacataaccg gacataatca taggacctct cacacacagt tcgcctcttt
      661 gattaacgcc cagcgttttc ccggtatcca gatccacaac cttcgcttca aaaaatggaa
      721 caactttacc gaccgcgccc ggtttatcat ccccctcggg tgtatcagaa tagctgatgt
      781 agtctcatga gccctatcct gctgatactg gcagatggaa acctcttggc aaccgcttcc
      841 cgactcttaa gaaggggacg ccacccgaac acattcctgt tgaataataa atacgtattt
      901 tcatt
//
