LOCUS       sequence-22318-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Klf4
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(150..191)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    244..268
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac
       61 cggaaccctt aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc
      121 tcgacgaatt gtcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg
      181 ataggcttac cttcgaaccg cgggccctct agactcgagc ggccgccact gtgctggata
      241 tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac ccttaaagtg cctcttcatg
      301 tgtaaggcaa ggtggtccga cctggaaaag gccctgtcgc acttctggca ctgaaagggc
      361 cggtgccctg tgtgtttgcg gtagtgcctg gtcagttcat ccgagcgggc gaatttccac
      421 ccacagccgt cccagtcaca gtggtaaggt ttctcgcctg tgtgagttcg caggtgtgcc
      481 ttgagatgag aactcttggt ataggttttg ccacagcctg catagtcaca agtgtgggtg
      541 gctgttcttt tccggggcca cgaccttctt cccctctttg gcttgggctc ctctggcagg
      601 caggaacccg gtggcatgag ctcttgataa tggagagagg ggacttgtga ctgcatct
//
