LOCUS       sequence-22320-e         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Oct4
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..119
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(157..198)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    251..275
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttccaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa ttgtacctag
       61 ctggaaccgg aacccttaaa catgtataac ttcgtataat gtatgctata cgaagttatt
      121 aggtccctcg acgaattgtc attactaacc ggtacgcgta gaatcgagac cgaggagagg
      181 gttagggata ggcttacctt cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg
      241 ctggatatca accactttgt acaagaaagc tgggtctaga tatctcgagc cccaaagctc
      301 caggttctct tgtctacctc ccttgccttg gctcacagca tccccaggga gggctggtgc
      361 ctcagtttga atgcatggga gagcccagag cagtgacggg aacagaggga aaggcctcgc
      421 cctcaggaaa agggactgag tagagtgtgg tgaagtgggg gcttccatag cctggggtgc
      481 caaagtgggg acctgggggc agaggaaagg atacagcccc cccctgggaa accctgtagc
      541 ctcatactct tctcgttggg a
//
