LOCUS       sequence-223209-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: pSpCas9(BB)-T2A-HygR
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        128..203
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        300..585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     gap             713..725
                     /estimated_length=13
ORIGIN
        1 tantttnant tttaaattta tnttttaaat ggactatcat atgcttaccg taacttgaaa
       61 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtcttcgag
      121 aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      181 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata
      241 aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc
      301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      361 acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      421 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      481 gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt
      541 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
      601 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
      661 attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggggg atnnnnnnnn
      721 nnnnnggggn gggggngngg nggggggnng gggnngggnn nggggnggnn ggggngnggg
      781 ggnnngnnnn nnggnggggg gggggngngg ggggggggnn ggnnnggggg gggggnggnn
      841 ngngggnggn ngggnnngnn ggnnngggng gngnngggng ggggg
//
