LOCUS       sequence-223239-        1485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRa negative control. Catalytically inactive Cas9 from S. 
            pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 EGFP-guide3
FEATURES             Location/Qualifiers
     source          1..1485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        147..165
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        217..235
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        322..533
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             550..597
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnttgnagt tttaaattat gttttaaatg gactatcata tgcttaccgt aacttgaaag
       61 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg atgccgttct
      121 tctgcttgtg ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca
      181 agttaaaata aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca
      241 gggccaagtg gcaccgagtc ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa
      301 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      361 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
      421 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      481 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggcgtacg
      541 gccaccatga aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac
      601 aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc
      661 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      721 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
      781 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
      841 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
      901 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
      961 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgaa aaagaaactg
     1021 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     1081 aagttccggg gccacttcct gatcgagggc gacctgaacc ccaacaacag caacttggac
     1141 aaactgttca tccagctggt ggcagaccta caaccagctt gttcagagaa aaccccatcc
     1201 acccccaccg gcgtggaacc ccaaggcctt cctgtctgcc aaactgaaca aaaacaaaaa
     1261 ggttggaaaa acttaatcgc ccacttcccc cgcggaaaaa aaaaaaaggc ctgttccccc
     1321 acccctgtat ggcctgaaac ctgggccctg caccccaact tttaaagaaa aactttacca
     1381 ttggccagag aagccaaatt acgggtttgg aaagaggaca ctccaaanac aaacctgnna
     1441 aatccggtgt ggccaattaa gggaaacnta ggccnaacaa ttgtt
//
