LOCUS       sequence-223245-        1258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2
            T...
FEATURES             Location/Qualifiers
     source          1..1258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        130..205
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        236..746
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     665..683
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      759..768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             795..977
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVCVDFTSEEWKLLDTAQQIVYRDVMLENYKNLVSLGY
                     QLTKPDVILRLEKEEE"
ORIGIN
        1 annttnnagt tttaaattat nttttaaaat ggactatcat atgcttaccg taacttgaaa
       61 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg cggaggaacg
      121 gagtagtctg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      181 tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct tgaaaggggt
      241 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
      301 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
      361 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
      421 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
      481 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
      541 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
      601 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
      661 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
      721 accgaatcac cgacctctct ccccaggacc ggtgatccgc caccatggat gctaagtcac
      781 taactgcctg gtcccggaca ctggtgacct tcaaggatgt atgtgtggac ttcaccagcg
      841 aggagtggaa gctgctggac actgctcagc agatcgtgta cagagatgtg atgctggaga
      901 actataagaa cctggtttcc ttgggttatc agcttactaa gccagatgtg atcctccggt
      961 tggagaagga agaagagctc ggcggttccg gcggagggtc gatggataag aaatacagca
     1021 tcggtctggc catcggnnac aattctgtcg gcngggccnn gatcaccgac aaatacaagg
     1081 ggccnaacga naaattccag ggggctggag gaacctgacc gggnaaaaat caagaagaac
     1141 ttgattgggc gcctgacgtt ccaaagngna gaaacggnta ntncccccgg ttgaaaaaat
     1201 ccctcaaaan aaaatccacc aacgaaaaac cggatctgtt ttcgcaaaaa tgttccaa
//
