LOCUS       sequence-223279-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 GLIS3 TSS-guide1
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..19
                     /estimated_length=18
     primer_bind     41..60
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        156..174
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        226..244
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gap             618..628
                     /estimated_length=11
     gap             630..640
                     /estimated_length=11
     gap             699..709
                     /estimated_length=11
ORIGIN
        1 annnnnnnnn nnnnnnnnnt ttnnnttatg ttttaaaatg gactatcata tgcttaccgt
       61 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      121 gctcgcgggt cactaggcgt tttagagcta ggccaacatg aggatcaccc atgtctgcag
      181 ggcctagcaa gttaaaataa ggctagtccg ttatcaactt ggccaacatg aggatcaccc
      241 atgtctgcag ggccaagtgg caccgagtcg gtgctttttt tggatcctag gtcttgaaag
      301 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      361 agaagttggg gggaggggtc ggcnattgat ccggtgccta gagaangngg cnngnantan
      421 nnngnnannn nnangncnng nnnnggntcc nncnttttcn nnanggnggg ngagannnnt
      481 atntnnnnnn ntnntnnncn ngnncnntct nannannatn nnnnnagtcg acnnnnnncn
      541 ngcatnnggn naanannnan aggnnnnnnn nnatnaaaaa cgttnnncan nnnannnnna
      601 nnnnnganna nnnnngcnnn nnnnnnnngn nnnnnnnnnn annnnnnntn nnttnnnnnn
      661 tgnnnannng ngnnnnttnt gtgnnnnnna gnnnnnnann nnnnnnnnnc nnnngnannc
      721 nnnncnnnnn nnnnannnnn nnnancnnaa nnngntnnnn nnnnnanncn nntnnnccna
      781 ntncnnna
//
