LOCUS       sequence-223293-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 CAMK1D TSS-guide4
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        143..161
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        213..231
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        318..529
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             546..593
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttnnagtttt aaattatgtt ttaaatggac tatcatatgc ttaccgtaac ttgaaagtat
       61 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggga gcgggacatc
      121 cccgagtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
      181 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
      241 caagtggcac cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg
      301 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      361 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      421 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      481 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca
      541 ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga
      601 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
      661 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
      721 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
      781 tgaagagaac cgccagaaga agatacacca gaacggaaga accgggatct gctattctgc
      841 aagagatctt tcggcaacga gatgggccaa ggtgggacga acggcttgct tccacagaac
      901 tggaaagagt ccgttcctgg tgggaagaag gataagaagg cagcgagacg ggcactccca
      961 tctttcgggc aaccttcgtg gtacgaaggt gagcctaccc acgagaaagt ttgcctatca
     1021 gttctagctg tctgaatgag ataaaactgg gtgggacctg tgatcggtac aagggcccga
     1081 aacttgccgg cttggatttt atct
//
