LOCUS       sequence-223313-        1766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs11257655-guide2
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..162
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        214..232
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        319..530
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             547..594
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnagtttta aattatnttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
       61 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggtag acacttgccc
      121 cgagtcgttt tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt
      181 taaaataagg ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg
      241 ccaagtggca ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg
      301 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      361 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      421 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      481 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc
      541 accatgaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggacaag
      601 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      661 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      721 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      781 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      841 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      901 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
      961 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     1021 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1081 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1141 ctgttcatcc agctggtgca aacctacaac cagctgttca aggaaaaccc atcaacgcag
     1201 cgggctggaa gccaaggcat cctggctgcc aactgaacaa agacaaaagg ctggaaaaat
     1261 tgatccccca cttccccggg aaaaaaaaaa gggccggttc cgaaccttat tgcccgaagc
     1321 cggggctgac cccccattta aaaaaaattt tcacttggcc gagaagccca attggcttta
     1381 aaaagggaac tctaaaaaaa caacggagaa cacctgtggg cacaaatgga caacataacc
     1441 aacatgtttt gtgcccaaaa aaatctcaga cacctctcgg gaagaacctc aggagagaca
     1501 acacaataca ggccgctcgn gccnctttta tgaaaaaaaa caccacaacc aggacctcgc
     1561 atagacnncg cngggcnggc tngaanaaaa taatttatnt ctacncaagg aangacgccg
     1621 cctaatggag gcacgngagn taactgtaac ttctctctgc aaaagggacg aggggtccaa
     1681 ggtaaggaaa gngaaaggcg ggacnncccc tctcccagaa gctctggtaa gtgtaatttg
     1741 ggagaagaat tcgcatacag cgttta
//
