LOCUS       sequence-22333-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p405CYC1
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     325..341
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     325..341
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(375..391)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      394..641
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(410..428)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(688..705)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(688..704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(697..719)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attaaccctc actaaaggga caaaagctgg agctcatttg gcgagcgttg gttggtggat
       61 caagcccacg cgtaggcaat cctcgagcag atccgccagg cgtgtatata tagcgtggat
      121 ggccaggcaa ctttagtgct gacacataca ggcatatata tatgtgtgcg acgacacatg
      181 atcatatggc atgcatgtgc tctgtatgta tataaaactc ttgttttctt cttttctcta
      241 aatattcttt ccttatacat taggaccttt gcagcataaa ttactatact tctatagaca
      301 cgcaaacaca aatacacaca ctaatctaga actagtggat cccccgggct gcaggaattc
      361 gatatcaagc ttatcgatac cgtcgacctc gagtcatgta attagttatg tcacgcttac
      421 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
      481 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
      541 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
      601 tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac ccaattcgcc
      661 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
      721 aa
//
