LOCUS       sequence-223454-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of RapGAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Liu C, Takahashi M, Li Y, Dillon TJ, Kaech S, Stork PJ
  TITLE     The interaction of Epac1 and Ran promotes Rap1 activation at the 
            nuclear envelope.
  JOURNAL   Mol Cell Biol. 2010 Aug;30(16):3956-69. doi: 10.1128/MCB.00242-10. 
            Epub 2010 Jun 14.
  PUBMED    20547757
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00242-10"; journalName: "Mol Cell Biol"; date: 
            "2010-08"; volume: "30"; issue: "16"; pages: "3956-69"
COMMENT     Sequence Label: Flag-RapGAP
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..75
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 aagctagagc tcggatccgc caccatggac tacaaggacg acgatgacaa ggactacaag
       61 gacgacgatg acaagaattc tattgagaag atgcagggaa gcaggatgga tgaacaacgc
      121 tgctccttcc cgccgcccct caaaacagag gaggactaca ttccataccc gagcgtgcac
      181 gaggtcttgg ggcgagaagg acccttcccc ctcatcctgc tgccccagtt tgggggctac
      241 tggattgagg gcaccaacca cgaaatcacc agcatccccg agacagagcc actgcagtcg
      301 cccacaacca aggtgaagct cgagtgcaac cccacagccc gcatctaccg gaagcacttt
      361 ctcggcaagg agcatttcaa ttactactca ctggacgctg ccctcggcca ccttgtcttc
      421 tcactcaagt acgatgtcat cggggaccaa gagcacctgc ggctgctgct caggaccaag
      481 tgccggacat accatgatgt catccccatc tcctgcctca ccgagttccc taatgttgtc
      541 cagatggcaa agttggtgtg tgaagacgtc aatgtggatc ggttctatcc tgtgctctac
      601 cccaaggctt cccggctcat cgtcaccttt gacgagcatg tcatcagcaa taacttcaag
      661 tttggcgtca tttatcagaa gcttgggcag acctccgagg aagaactctt cagcaccaat
      721 gaggaaagtc ccgctttcgt ggagttcctt gaatttcttg gccagaaggt caaactgcag
      781 gactttaagg ggttccgagg aggcctggac gtgacccacg ggcagacggg gaccgaatct
      841 gtgtactgca acttccgcaa caaggagatc atgtttcacg tgtccaccaa gctgccatac
      901 acggaagggg acgcccagca gttgcagcgg aagcggcaca tcgggaacga catcgtggct
      961 gtggtcttcc aggatgaaaa cactcctttc gtgcccgaca tgatcgcgtc caacttcctg
     1021 catgcctacg tcgtg
//
