LOCUS       sequence-223615-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Du X, Tabeta K, Hoebe K, Liu H, Mann N, Mudd S, Crozat K, Sovath S, 
            Gong X, Beutler B
  TITLE     Velvet, a dominant Egfr mutation that causes wavy hair and defective
            eyelid development in mice.
  JOURNAL   Genetics. 2004 Jan . 166(1):331-40.
  PUBMED    15020428
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2004 Jan . 166(1):331-40."
COMMENT     Sequence Label: pFLAG-Egfr(Velvet)
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             802..846
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             847..870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4473..5095
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4672..4691)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5119..5139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5137..5435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5489..6283
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5543..5562)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6153..6172
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6935..7069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6972..6991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7026..7045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7074..7093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7075..7093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7107..7123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7107..7123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7131..7147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7155..7161,7162..7179,7180..7185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7200..7221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7338..7355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7509..8097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7589..7608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8268..9059,9060..9128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8891..8910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9129..9233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9347..9366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9574..9595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(9858..9872,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9872,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attaattcag atcgatctac catgtctgca cttctgatcc tagctcttgt tggagctgca
      841 gttgctgact acaaagacga tgacgacaag cttttggagg aaaagaaagt ctgccaaggc
      901 acaagtaaca ggctcaccca actgggcact tttgaagacc actttctgag cctgcagagg
      961 atgtacaaca actgtgaagt ggtccttggg aacttggaaa ttacctatgt gcaaaggaat
     1021 tacgaccttt ccttcttaaa gaccatccag gaggtggccg gctatgtcct cattgccctc
     1081 aacaccgtgg agagaatccc tttggagaac ctgcagatca tcaggggaaa tgctctttat
     1141 gaaaacacct atgccttagc catcctgtcc aactatggga caaacagaac tgggcttagg
     1201 gaactgccca tgcggaactt acaggaaatc ctgattggtg ctgtgcgatt cagcaacaac
     1261 cccatcctct gcaatatgga tactatccag tggagggaca tcgtccaaaa cgtctttatg
     1321 agcaacatgt caatggactt acagagccat ccgagcagtt gccccaagtg tgatccaagc
     1381 tgtcccaatg gaagctgctg gggaggagga gaggagaact gccagaaatt gaccaaaatc
     1441 atctgtgccc agcaatgttc ccatcgctgt cgtggcaggt cccccagtga ctgctgccac
     1501 aaccaatgtg ctgcggggtg tacagggccc cgagagagtg actgtctggt ctgccaaaag
     1561 ttccaagatg aggccacatg caaagacacc tgcccaccac tcatgctgta caaccccacc
     1621 acctatcaga tggatgtcaa ccctgaaggg aagtacagct ttggtgccac ctgtgtgaag
     1681 aagtgccccc gaaactacgt ggtgacagat catggctcat gtgtccgagc ctgtgggcct
     1741 gactactacg aagtggaaga agatggcatc cgcaagtgta aaaaatgtga tgggccctgt
     1801 cgcaaagttt gtaatggcat aggcattggt gaatttaaag acacactctc cataaatgct
     1861 acaaacatca agcacttcaa atactgcact gccatcagcg gggaccttca catcctgcca
     1921 gtggccttta agggggattc tttcacgcgc actcctcctc tagacccacg agaactagaa
     1981 attctaaaaa ccgtaaagga aataacaggc tttttgctga ttcaggcttg gcctgataac
     2041 tggactgacc tccatgcttt cgagaaccta gaaataatac gtggcagaac aaagcaacat
     2101 ggtcagtttt ctttggcggt cgttggcctg aacatcacat cactggggct gcgttccctc
     2161 aaggagatca gtgatgggga tgtgatcatt tctggaaacc gaaatttgtg ctacgcaaac
     2221 acaataaact ggaaaaaact cttcgggaca cccaatcaga aaaccaaaat catgaacaac
     2281 agagctgaga aagactgcaa ggccgtgaac cacgtctgca atcctttatg ctcctcggaa
     2341 ggctgctggg gccctgagcc cagggactgt gtctcctgcc agaatgtgag cagaggcagg
     2401 gagtgcgtgg agaaatgcaa catcctggag ggggaaccaa gggagtttgt ggaaaattct
     2461 gaatgcatcc agtgccatcc agaatgtctg ccccaggcca tgaacatcac ctgtacaggc
     2521 aggggaccag acaactgcat ccagtgtgcc cactacattg atggcccaca ctgtgtcaag
     2581 acctgcccag ctggcatcat gggagagaac aacactctgg tctggaagta tgcagatgcc
     2641 aataatgtct gccacctatg ccacgccaac tgtacctatg gatgtgctgg gccaggtctt
     2701 caaggatgtg aagtgtggcc atctgggcca aagataccat ctattgccac tgggattgtg
     2761 ggtggcctcc tcttcatagt ggtggtggcc cttgggattg gcctattcat gcgaagacgt
     2821 cacattgttc gaaagcgtac actacgccgc ctgcttcaag agagagagct cgtggaacct
     2881 ctcacaccca gcggagaagc tccaaaccaa gcccacttga ggatattaaa ggaaacagaa
     2941 ttcaaaaaga tcaaagttct gggttcggga gcatttggca cagtgtataa gggtctctgg
     3001 atcccagaag gtgagaaagt aaaaatcccg gtggccatca aggagttaag agaagccaca
     3061 tctccaaaag ccaacaaaga aatccttgac gaagcctatg tgatggctag tgtggacaac
     3121 cctcatgtat gccgcctcct gggcatctgt ctgacctcca ctgtccagct cattacacag
     3181 ctcatgccct acggttgcct cctggactac gtccgagaac acaaggacaa cattggctcc
     3241 cagtacctcc tcaactggtg tgtgcagatt gcaaagggca tgaactacct ggaagatcgg
     3301 cgtttggtgc accgtgactt ggcagccagg aatgtactgg tgaagacacc acagcatgtc
     3361 aagatcacag gttttgggct ggccaaactg cttggtgctg aagagaaaga atatcatgcc
     3421 gaggggggca aagtgcctat caagtggatg gctttggaat caattttaca ccgaatttat
     3481 acacaccaaa gtgatgtctg gagctatggt gtcactgtgt gggaactgat gacctttggg
     3541 tccaagcctt atgatggaat cccagcaagt gacatctcat ccatcctaga gaaaggagag
     3601 cgccttccac agccacctat ctgcaccatc gatgtctaca tgatcatggt caagtgctgg
     3661 atgatagatg ctgatagccg cccaaagttc cgagagttga ttcttgaatt ctccaaaatg
     3721 gcccgagacc cacagcgcta ccttgttatc cagggggatg aaagaatgca tttgccaagc
     3781 cctacagact ccaactttta ccgagccctg atggatgaag aggacatgga ggatgtagtt
     3841 gatgctgatg agtatcttat cccacagcaa ggcttcttca acagcccgtc cacgtcgagg
     3901 actcccctct tgagttctct gagtgcaact agcaacaatt ccactgtggc ttgcattaat
     3961 agaaatggga gctgccgtgt caaagaagac gccttcttgc agcggtacag ctccgacccc
     4021 acaggtgctg taacagagga caacatagat gacgcattcc tccctgtacc tgaatatgta
     4081 aaccaatctg ttcccaagag gccagcaggc tctgtgcaga accctgtcta tcacaatcag
     4141 cccctgcatc cagctcctgg aagagacctg cattatcaaa atccccacag caatgcagtg
     4201 ggcaaccctg agtatctcaa cactgcccag cctacctgtc tcagtagtgg gtttaacagc
     4261 cctgcactct ggatccagaa aggcagtcac caaatgagcc tagacaaccc tgactaccag
     4321 caggacttct tccccaagga aaccaagcca aatggcatat ttaagggccc cacagctgaa
     4381 aatgcagagt acctacgggt ggcacctcca agcagcgagt ttattggagc atgaagatct
     4441 gatatcggta ccagtcgact ctagaggatc ccgggtggca tccctgtgac ccctccccag
     4501 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     4561 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     4621 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     4681 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     4741 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     4801 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     4861 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     4921 cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac cagcaggagg
     4981 acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat ttgagttgct
     5041 tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt gaattgggta
     5101 cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5281 tagtcccgcc cctaactccg cccatcccgc ctccgctaac tccgcccagt tccgcccatt
     5341 ctccgcccca tggctgacta atttttttta tttatgcagg ccgaggccgc ctcggcctct
     5401 gagctattcc agaagtagtg aggaggcttt tttggaggaa ttgatcagct tgggatctga
     5461 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5521 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5581 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5641 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5701 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5761 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5821 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5881 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5941 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6001 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6061 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6121 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6181 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6241 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6301 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6361 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6421 cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg
     6481 ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc
     6541 ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg
     6601 ggaggcacga tggccgcttt ggtcgacccg gacgggacgc tcctgcgcct gatacagaac
     6661 gaattgcttg caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt
     6721 ggatggagcg ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgccaagct
     6781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgccga tcataatcag
     6841 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     6901 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     6961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca attccctata
     7081 gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     7141 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7201 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7261 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7321 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7381 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7441 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7501 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7561 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7621 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7681 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7741 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7801 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7861 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7921 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7981 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8041 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8101 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8161 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8221 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8281 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8341 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8401 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8461 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8521 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8581 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8641 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8701 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8761 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8821 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8881 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8941 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9001 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9061 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9241 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9301 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9481 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9541 tccacgttct ttaagagtcc actattaaag aacgtggact cttgttccaa actggaacaa
     9601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     9661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9721 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9781 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9841 ggtaacgcca gggttttccc agtcacgacg tt
//
