LOCUS       sequence-223899-        3259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab1bb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab1bb
FEATURES             Location/Qualifiers
     source          1..3259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..96
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    716..840
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(911..928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(911..927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(920..942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      991..1077
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1169..1196
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1355..1372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1606..1625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2261..3070)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2984..3003
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3062..3081
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3183..3199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3183..3199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3204..3223
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3204..3222
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
       61 gcttttttat aatgccaact ttgtataata aagttggaaa acaagagttt tctaacagca
      121 gccgccgcct gactgttgca ccggggtgct ttcgatttta aggtcaggtt tctcagagcc
      181 ccctgaagct cctggtctca tgcgcttctt tatctcctct gccatggtca tgaaggcctg
      241 ctccacgttt gtggcatttt tggcactcgt ctcaaggaaa gggatgccaa gagagtcagc
      301 aaattcctta gctgtagtgt agtccacaac cttttttgta gtcaggtcac acttatttcc
      361 aacaagcagt ttgttgacat tttcactcgc atagcgatcg atttccttta gccactgttt
      421 cacattgttg taggattcct gatctgtaac atcatataca acgatgatcc catgagcgcc
      481 cctgtagtag ctggatgtaa tagtgcggaa tctctcctgt ccagccgtgt cccatatttg
      541 cagtttaata gtttttccat ccaactcaat ggttcggatc ttaaaatcca ctccaattgt
      601 gctgatgaaa ctctccgtgt aggtgtcgtc agcaaaacgc aacaaaatgc aggattttcc
      661 cactccagaa tctccaatca gaagaagctt gaataagtag tcatattcag ggttcaccac
      721 tttgtacaag aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      781 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
      841 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcc
      901 cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg
      961 ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac
     1021 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1081 ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg
     1141 ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg
     1201 tttctacaaa ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg
     1261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2161 acgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg
     2221 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     2281 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2341 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2401 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2461 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2521 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     2581 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     2641 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     2701 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     2761 ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     2821 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2881 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2941 tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3001 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa
     3061 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     3121 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct
     3181 gccaggaaac agctatgacc atgtaatacg actcactata ggggatatcg cggccgcgtt
     3241 aacgctacca tggagctcc
//
