LOCUS       sequence-223960-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Liu CT, Hanoian P, French JB, Pringle TH, Hammes-Schiffer S, 
            Benkovic SJ
  TITLE     Functional significance of evolving protein sequence in 
            dihydrofolate reductase from bacteria to humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10159-64. doi: 
            10.1073/pnas.1307130110. Epub 2013 Jun 3.
  PUBMED    23733948
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307130110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-06-18- 18"; volume: "110"; issue: "25"; pages: 
            "10159-64"
COMMENT     Sequence Label: pET22b-ecDHFR-N23PP/L28F/G51PEKN
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             548..565
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(618..636)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      632..679
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gatatacata tgatcagtct gattgcggcg ctagcggtag atcgcgttat cggcatggaa
       61 aacgccatgc catggccgcc gctgcctgcc gatttcgcct ggtttaaacg caacacctta
      121 aataaacccg tgattatggg gcgccatacc tgggaatcaa tcccagaaaa aaacaggcct
      181 ttgcccggcc gcaaaaatat tatcctcagc agtcaacccg ggaccgatga tcgggttacc
      241 tgggttaaat cggtcgacga agccatcgcg gcgtgtggtg acgtaccaga aatcatggtg
      301 attggcggcg gacgcgttta tgaacagttc ttgccaaaag cgcaaaaact gtatctgacg
      361 catatcgatg cagaagtgga aggcgacacc cattttccgg attacgagcc ggatgactgg
      421 gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga actcgcatag ctattgtttc
      481 gaaatcctcg agcgtcgtta aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      541 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      601 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      661 ggtcttgagg ggttttttgc tgaaag
//
