LOCUS       sequence-223965-        3244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab30
FEATURES             Location/Qualifiers
     source          1..3244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(730..825)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(862..881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(863..881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(886..902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(886..902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1004..1023)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1015..1824
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1082..1101)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1971..2559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2460..2479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2713..2730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2889..2916)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3008..3094)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3143..3165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3157..3174
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3158..3174
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggagg attatgatta cttattcaaa attgtgctga taggaaatgc aggagtgggt
      181 aaaacctgcc tggtgcgacg tttcactcag gggctgtttc ctcctggaca gggtgccaca
      241 attggagttg attttatgat taaaactgtg gaaataaaag gagttaaggt gaagctccag
      301 atttgggaca cagctggtca agaaagattt cgctctataa ctcagagcta ctaccgcagt
      361 gccaatgccc tgatcctcac ctacgacatc acctgcgaag attcgttccg gtgccttcct
      421 gagtggctga gggagattga gcagtacgcc aacaaccaag tggtgacaat ccttgtaggg
      481 aataagatag acttggctga taagcgagaa gtccttcgtc aacgagcaga ggagtttgca
      541 gattctcaga gcatgcttta tctggagacc tctgccaaag aatcagacaa cgtggagaag
      601 ctctttctag atctggcctg cgaactaatc cgtgaggcca agcaaaacac gttggacaac
      661 aacgactcca ctcccatgcc cggagaaggg aaaaacatca actatttggg ctgctgcagc
      721 atgaactagc aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt
      781 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag
      841 cgttaacgcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
      901 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      961 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1021 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     1321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     1441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     1501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     1561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     1681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     1741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     1801 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     1861 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     1921 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2761 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2821 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     2881 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     2941 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3001 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3061 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3121 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     3181 ctcgggccct gcagctctag agctcgaatt ctacaggtca ctaataccat ctaagtagtt
     3241 ggtt
//
