LOCUS       sequence-223972-        3244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab34b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab35a
FEATURES             Location/Qualifiers
     source          1..3244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..96
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    701..825
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(896..913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(896..912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(905..927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      976..1062
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1154..1181
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1340..1357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1511..2099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1591..1610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2246..3055)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2969..2988
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3047..3066
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3168..3184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3168..3184
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3189..3208
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3189..3207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
       61 gcttttttat aatgccaact ttgtataata aagttgctag cagcacttct tcttccgttt
      121 gctgttctgc ttgagtttaa tcacatcgtt ctgctgctgc tgctgctgtt tggccacaga
      181 ctctttcttt gcccggagaa ccaactctgt gatgcagtta aacatctcct ccacattgac
      241 attttctttc gcacttgtct cgaacagact gatgcccatc tgttctgcga acttctgtgc
      301 atcattcgtc tccaccacct tagagtttgg atcatcattt ttattgccca ctaatattcg
      361 acagacgtca tcacagttct ggtttatttc atgtagccat cgtttaacat tgacaaagga
      421 ctcggcgctg gtaacgtcat acactactat cacaccatgg gttcctctgt aatatgtaga
      481 tgtaatcgtt cggaatcgct cttgtccggc tgtgtcccat atctgcagtt tcaccttctc
      541 tccattaagc tccacagtgc ggatcttgaa gtcgactcct atagtggtga tgtaactgcc
      601 tgaaaatgtg ttgtcggcga aacgcagaag aagactgctc tttccaacac cgctgtcacc
      661 gatgatcagc agcttgaaga ggtgatcgta gtcgcgggcc accactttgt acaagaaagt
      721 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      781 acagactaca taatactgta aaacacaaca tatgcagtca ctatgaacca actacttaga
      841 tggtattagt gacctgtaga attcgagctc tagagctgca gggcccgagc ttaagactgg
      901 ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag
      961 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
     1021 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     1081 aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     1141 catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     1201 cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg taactcacgt
     2161 taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgccagt
     2221 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     2281 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     2341 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     2401 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     2461 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     2521 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     2581 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     2641 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     2701 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga
     2761 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     2821 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     2881 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat
     2941 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     3001 catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac
     3061 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     3121 cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgccag gaaacagcta
     3181 tgaccatgta atacgactca ctatagggga tatcgcggcc gcgttaacgc taccatggag
     3241 ctcc
//
