LOCUS       sequence-223983-        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab3c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab3c
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(692..787)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(824..843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(825..843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(848..864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(848..864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(966..985)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             977..1786
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1044..1063)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1933..2521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2422..2441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2675..2692
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2851..2878)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2970..3056)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3105..3127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3119..3136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3120..3136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggctg ctacgcaaga caacaaacaa aaggagaact
       61 ctgatcaaaa ttttgattac atgttcaaat tgctgatcat cgggaacagc agcgtcggga
      121 aaacatcctt cctcttccga tacgctgatg ataccttcac atctgctttt gtcagtacag
      181 tgggcatcga ctttaaggtc aaaactgtct acaagaacga caagaggatc aagcttcaga
      241 tctgggacac agccggacag gaacgataca ggactatcac cactgcctac tacagagggg
      301 ccatgggctt catcctaatg tatgacatta ctaacgaaga gtcttttgct gctgtacagg
      361 actgggcgac ccagattaag acatactcgt gggacaatgc acaggtgatc ctcgctggaa
      421 acaaatgtga catggaggag gagaggatag tgtctgtgga cagcggcaga ctgctggcag
      481 aacagctcgg atttgagttt ttcgagacca gtgcgaagga caacatcaac gtgaagcaga
      541 cttttgaccg ccttgtggat ataatctgcg acaagatgtc cgacactctg gaggccgatc
      601 ctgcggcggc cggcgggact cccagcgcca gactgaccga gacccctgca ccccctcagc
      661 cgtcagactg cagctgctga tcggctcaac ccaactttat tatacaaagt tggcattata
      721 aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      781 attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta
      841 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      901 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      961 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1021 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1081 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1141 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1201 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1261 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1321 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1381 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1441 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1501 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1561 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1621 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1681 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1741 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     1801 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1861 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     1921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2041 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2581 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2641 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2701 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2761 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2821 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     2881 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     2941 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     3001 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     3061 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
     3121 taaaacgacg gccagtctta agctcgggcc ctgcagctct agagctcgaa ttctacaggt
     3181 cactaatacc atctaagtag ttggttcata gtgactgcat atgttgtgtt ttacagtatt
     3241 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3301 tttctc
//
