LOCUS       sequence-224042-        3262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab8a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab8a
FEATURES             Location/Qualifiers
     source          1..3262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(748..843)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(880..899)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(881..899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(904..920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(904..920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1022..1041)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1033..1842
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1100..1119)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1989..2577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2478..2497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2731..2748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2907..2934)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3026..3112)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3161..3183
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3175..3192
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3176..3192
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcga agacctacga ttatttgttt aaactgctgt taatcgggga ttccggagtc
      181 gggaagacct gtgtgctgtt cagattctca gaggatgcct ttaactcgac atttatctcg
      241 acgataggta tcgatttcaa gatcaggaca atagaactag atggcaagaa gataaagtta
      301 cagatatggg acacggcagg acaggaacga tttcggacga tcacgacagc atattacaga
      361 ggtgcaatgg gtattatgct ggtttatgat atcaccaatg agaagtcatt tgacaacatc
      421 aaaaactgga tccgaaacat agaggagcat gcatcagctg atgtggaaaa gatgattttg
      481 gggaacaaat gtgacattaa tgagaagagg caggtgtcta aagacagagg cgagaagctg
      541 gcgttagagt acggcatcaa gttcatggag acaagtgcaa aggctaatat caacgttgag
      601 aatgctttct taacactcgc cagagacata aaagcaaaga tggacacaaa attggaggga
      661 aacaatcctc agggcagcaa ccacggtgtg aaaattacca ctgaacagcc gaaaaagagc
      721 agcttctttc gctgtgtgct actgtgacaa ctttattata caaagttggc attataaaaa
      781 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      841 tttggagctc catggtagcg ttaacgcggc cgcgatatcc cctatagtga gtcgtattac
      901 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
      961 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1021 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1081 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1141 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1201 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1261 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1321 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1381 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1441 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1501 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1561 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1621 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1681 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1741 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1801 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     1861 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     1921 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2701 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2761 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2821 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     2881 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     2941 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3001 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     3061 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     3121 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     3181 acgacggcca gtcttaagct cgggccctgc agctctagag ctcgaattct acaggtcact
     3241 aataccatct aagtagttgg tt
//
