LOCUS       sequence-224047-        3258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab9b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab9b
FEATURES             Location/Qualifiers
     source          1..3258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(644..739)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(776..795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(777..795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(800..816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(800..816)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(918..937)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             929..1738
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(996..1015)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1885..2473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2374..2393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2627..2644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2803..2830)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2922..3008)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3057..3079
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3071..3088
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3072..3088
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgagtg ggaagagcct cttgctgaag gtcattcttc
       61 tgggggatgg cggcgtgggc aaaagctccc tcatgaaccg atatgtgacg gaccgcttcg
      121 actcgcagtc tttccatacc atcggtgtcg aattccttaa ccgcgacctc gaaatcgatg
      181 gtcggctggt caccctacag atctgggaca cggcaggcca ggagcgattc aagagcctgc
      241 gcacaccttt ttaccgtggt gcagattgct gtctgctcac gttcgcagtg gatgacctgc
      301 agagcttcca gaatctgggc tgctggaaaa aggagttcat gtattattca gatgtacgag
      361 acccggagcg cttccccttt gtagtgctgg gaaacaaggt agacaaggaa gagcgtgagg
      421 tcggtgagga tgaggccagg gcctggtgtg aagaaaatgg ctgctgtccg tactttgaaa
      481 ccagtgcaaa ggacgacaca aatgtggggg ctgctttcga agccgctgtt cgggaggttc
      541 tggctagcga ggaccccatt gaccacacct tgctgagcag ttccatcgat ctccatggaa
      601 accgtaaagt gacccgctcg tcctgctgtt gaggtgcctc taacaacttt attatacaaa
      661 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      721 caaaataaaa tcattatttg gagctccatg gtagcgttaa cgcggccgcg atatccccta
      781 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
      841 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      901 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
      961 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1021 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     1081 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1141 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1201 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     1261 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1321 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     1381 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1441 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     1501 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1561 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1621 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1681 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     1741 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     1801 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     1861 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1921 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1981 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2041 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2101 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2161 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2221 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2281 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2341 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2401 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2461 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2521 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2581 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2641 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2701 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2761 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     2821 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     2881 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     2941 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     3001 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     3061 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccctgcagct ctagagctcg
     3121 aattctacag gtcactaata ccatctaagt agttggttca tagtgactgc atatgttgtg
     3181 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     3241 tatcatttta cgtttctc
//
