LOCUS       sequence-22405-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Jen Y, Manova K, Benezra R
  TITLE     Expression patterns of Id1, Id2, and Id3 are highly related but 
            distinct from that of Id4 during mouse embryogenesis.
  JOURNAL   Dev Dyn. 1996 Nov . 207(3):235-52.
  PUBMED    8922523
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            1996 Nov . 207(3):235-52."
COMMENT     Sequence Label: pBS/KS-mId1sp
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(397..413)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(398..414)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(442..460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(481..497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(481..497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(494..516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    505..521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(529..535,536..553,554..559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    574..595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(712..729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggccgctct agactagtgg atcctgaact cggagtctga agtcgggacc accggaggcc
       61 ggggactgcc tgtccgcgcc ccgctcagca ccctgaacgg cgagatcagt gccttggcgg
      121 ccgaggcggc atgtgttcca gccgacgatc gcatcttgtg tcgctgaggc ggcgcactga
      181 gggaccagat ggactccagc ccttcaggag gcaagaggaa aaaagtgctc tcggttcccc
      241 aggggatctc tgggaaagac actaccgcag ccaccggact cttggcggat cggtccagtg
      301 ggtagagggt ttgatcaaca gagcctcacc ctctccacct ttcagcctcc agagactttg
      361 gggagggggt taatcagaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
      421 gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
      481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
//
